| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2019-01-17 |
| タイトル |
|
|
タイトル |
COMPARISON OF WESTERN BLOT ANALYSES IN NATIVE AND ACID-ETHANOL-EXTRACTED SERUM FOR DETECTING BIG IGF-II IN PATIENTS WITH NON-ISLET CELL TUMOR HYPOGLYCEMIA |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Insulin-like growth factor-II |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Western blot |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Acid-ethanol extraction |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Non-islet cell tumor hypoglycemia |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Recurrent hypoglycemia |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| ID登録 |
|
|
ID登録 |
10.20569/00003691 |
|
ID登録タイプ |
JaLC |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 作成者 |
Kashima, Riko
Fujita, Hiroki
Otomo, Hitomi
Fukuoka, Yuki
Yamada, Yuichiro
|
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Non-islet cell tumor hypoglycemia (NICTH) triggered by high molecular weight insulin-like growth factor (IGF)-II (big IGF-II) is a rare cause of recurrent hypoglycemia. Western blot analysis is thought to be a best method to detect the presence of the big IGF-II in serum or tumor of patients with NICTH. However, its detailed protocols regarding treatment of human serum samples have not been reported. To determine how human serum samples should be treated in this analysis, we performed western blot analyses for detecting big IGF-II in native and acid-ethanolextracted serum samples obtained from three patients with recurrent hypoglycemia and hypoinsulinemia who were clinically diagnosed as NICTH. In abdominal computed tomography examination, bulky tumor which occupies abdominopelvic cavity was found in two cases of these patients, and renal tumor was detected in the other case. Through western blot analyses in these three cases, we found that big IGF-II is more clearly visualized in acid-ethanol-extracted serum rather than native serum. The present results suggest that the acid-ethanol-extraction method in western blot analysis for detecting big IGF-II would facilitate the diagnosis of big IGFII producing NICTH. |
|
言語 |
en |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 書誌情報 |
ja : 秋田医学
en : Akita journal of medicine
巻 45,
号 2,
p. 45-50,
発行日 2018-11-30
|
| 収録物識別子 |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0386-6106 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00009294 |
| 出版者 |
|
|
出版者 |
秋田医学会 |
|
言語 |
ja |
akitai45_2(45) (700.4 kB)
