{"created":"2023-07-25T10:23:36.617638+00:00","id":3331,"links":{},"metadata":{"_buckets":{"deposit":"9d460c40-113b-482f-baf0-78fb666f9326"},"_deposit":{"created_by":18,"id":"3331","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"3331"},"status":"published"},"_oai":{"id":"oai:air.repo.nii.ac.jp:00003331","sets":["611:862:863:1176"]},"author_link":["7586","11239","11240","6611","11241"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-11-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"50","bibliographicPageStart":"45","bibliographicVolumeNumber":"45","bibliographic_titles":[{"bibliographic_title":"秋田医学"},{"bibliographic_title":"AKITA JOURNAL OF MEDICINE","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"内容記述（抄録）","attribute_value_mlt":[{"subitem_description":"Non-islet cell tumor hypoglycemia (NICTH) triggered by high molecular weight insulin-like growth factor (IGF)-II (big IGF-II) is a rare cause of recurrent hypoglycemia. Western blot analysis is thought to be a best method to detect the presence of the big IGF-II in serum or tumor of patients with NICTH. However, its detailed protocols regarding treatment of human serum samples have not been reported. To determine how human serum samples should be treated in this analysis, we performed western blot analyses for detecting big IGF-II in native and acid-ethanolextracted serum samples obtained from three patients with recurrent hypoglycemia and hypoinsulinemia who were clinically diagnosed as NICTH. In abdominal computed tomography examination, bulky tumor which occupies abdominopelvic cavity was found in two cases of these patients, and renal tumor was detected in the other case. Through western blot analyses in these three cases, we found that big IGF-II is more clearly visualized in acid-ethanol-extracted serum rather than native serum. The present results suggest that the acid-ethanol-extraction method in western blot analysis for detecting big IGF-II would facilitate the diagnosis of big IGFII producing NICTH.","subitem_description_type":"Other"}]},"item_10001_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.20569/00003691","subitem_identifier_reg_type":"JaLC"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"秋田医学会"}]},"item_10001_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AN00009294","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03866106","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kashima, Riko "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujita, Hiroki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Otomo, Hitomi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukuoka, Yuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamada, Yuichiro"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-01-17"}],"displaytype":"detail","filename":"akitai45_2(45).pdf","filesize":[{"value":"700.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"akitai45_2(45)","url":"https://air.repo.nii.ac.jp/record/3331/files/akitai45_2(45).pdf"},"version_id":"fead5d25-956f-4acf-9c40-f92a04956855"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Insulin-like growth factor-II","subitem_subject_scheme":"Other"},{"subitem_subject":"Western blot","subitem_subject_scheme":"Other"},{"subitem_subject":"Acid-ethanol extraction","subitem_subject_scheme":"Other"},{"subitem_subject":"Non-islet cell tumor hypoglycemia","subitem_subject_scheme":"Other"},{"subitem_subject":"Recurrent hypoglycemia","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"COMPARISON OF WESTERN BLOT ANALYSES IN NATIVE AND  ACID-ETHANOL-EXTRACTED SERUM FOR DETECTING  BIG IGF-II IN PATIENTS WITH  NON-ISLET CELL TUMOR HYPOGLYCEMIA","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"COMPARISON OF WESTERN BLOT ANALYSES IN NATIVE AND  ACID-ETHANOL-EXTRACTED SERUM FOR DETECTING  BIG IGF-II IN PATIENTS WITH  NON-ISLET CELL TUMOR HYPOGLYCEMIA"}]},"item_type_id":"10001","owner":"18","path":["1176"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-01-17"},"publish_date":"2019-01-17","publish_status":"0","recid":"3331","relation_version_is_last":true,"title":["COMPARISON OF WESTERN BLOT ANALYSES IN NATIVE AND  ACID-ETHANOL-EXTRACTED SERUM FOR DETECTING  BIG IGF-II IN PATIENTS WITH  NON-ISLET CELL TUMOR HYPOGLYCEMIA"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-25T11:22:23.034614+00:00"}