@article{oai:air.repo.nii.ac.jp:00003331,
 author = {Kashima, Riko and Fujita, Hiroki and Otomo, Hitomi and Fukuoka, Yuki and Yamada, Yuichiro},
 issue = {2},
 journal = {秋田医学, AKITA JOURNAL OF MEDICINE},
 month = {Nov},
 note = {Non-islet cell tumor hypoglycemia (NICTH) triggered by high molecular weight insulin-like growth factor (IGF)-II (big IGF-II) is a rare cause of recurrent hypoglycemia. Western blot analysis is thought to be a best method to detect the presence of the big IGF-II in serum or tumor of patients with NICTH. However, its detailed protocols regarding treatment of human serum samples have not been reported. To determine how human serum samples should be treated in this analysis, we performed western blot analyses for detecting big IGF-II in native and acid-ethanolextracted serum samples obtained from three patients with recurrent hypoglycemia and hypoinsulinemia who were clinically diagnosed as NICTH. In abdominal computed tomography examination, bulky tumor which occupies abdominopelvic cavity was found in two cases of these patients, and renal tumor was detected in the other case. Through western blot analyses in these three cases, we found that big IGF-II is more clearly visualized in acid-ethanol-extracted serum rather than native serum. The present results suggest that the acid-ethanol-extraction method in western blot analysis for detecting big IGF-II would facilitate the diagnosis of big IGFII producing NICTH.},
 pages = {45--50},
 title = {COMPARISON OF WESTERN BLOT ANALYSES IN NATIVE AND  ACID-ETHANOL-EXTRACTED SERUM FOR DETECTING  BIG IGF-II IN PATIENTS WITH  NON-ISLET CELL TUMOR HYPOGLYCEMIA},
 volume = {45},
 year = {2018}
}