| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2016-08-01 |
| タイトル |
|
|
タイトル |
RAPID IMMUNOHISTOCHEMISTRY OF IDH-1 FOR THE INTRAOPERATIVE DIAGNOSIS OF GLIOMAS |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
IDH-1 |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
rapid immunohistochemistry |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
glioma |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
intraoperative diagnosis |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
DNA sequencing |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 作成者 |
HIROSHIMA, Yuko
NANJO, Hiroshi
SASAJIMA, Toshio
SHIMIZU, Hiroaki
MINAMIYA, Yoshihiro
YOSHIOKA, Toshiaki
ODA, Masaya
KUDO-ASABE, Yukitsugu
TSUDA, Masumi
TANINO, Mishie
TANAKA, Shinya
AKAGAMI, Yoichi
GOTO, Akiteru
|
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Immunohistochemistry (IHC) plays a major role in the histopathological diagnosis of central nervous system (CNS) tumors. We developed a rapid immunohistochemistry (R-IHC) method using an alternating current electric field, which enables the completion of IHC in 20 min. We performed intraoperative R-IHC on 16 glioma patients (9 with grade I-III gliomas and 7 with glioblastomas) using the anti-mutant isocitrate dehydrogenase 1 (IDH1) monoclonal antibody (IDH1- R132H ; H09), and the results were validated on permanent formalin-fixed paraffin-embedded samples of the same patients by standard IHC. DNA sequencing of the IDH1 gene was also performed for the 16 patients. Of 6 gliomas, 5 were shown to be positive by both R-IHC and standard IHC, and IDH1 mutation was confirmed by DNA sequencing. According to the results of R-IHC compared with both standard IHC results and DNA sequencing, the sensitivity of IDH1 R-IHC was 83% (5/6) ; specificity, 70% (7/10) ; positive predictive value, 63% (5/8) ; negative predictive value, 88% (7/8) ; and accuracy, 75% (12/16). Thus, R-IHC using the anti-mutant IDH1 monoclonal antibody has potential applications in the intraoperative identification of IDH1 mutations in gliomas. |
|
言語 |
en |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 書誌情報 |
ja : 秋田医学
巻 42,
号 3/4,
p. 147-156,
発行日 2016-03-31
|
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
03866106 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00009294 |
| 出版者 |
|
|
出版者 |
秋田医学会 |
|
言語 |
ja |
akitai42-3_4(147).pdf (1.0 MB)
