@article{oai:air.repo.nii.ac.jp:00002775,
 author = {HIROSHIMA, Yuko and NANJO, Hiroshi and SASAJIMA, Toshio and SHIMIZU, Hiroaki and MINAMIYA, Yoshihiro and YOSHIOKA, Toshiaki and ODA, Masaya and KUDO-ASABE, Yukitsugu and TSUDA, Masumi and TANINO, Mishie and TANAKA, Shinya and AKAGAMI, Yoichi and GOTO, Akiteru},
 issue = {3/4},
 journal = {秋田医学},
 month = {Mar},
 note = {Immunohistochemistry (IHC) plays a major role in the histopathological diagnosis of central nervous
system (CNS) tumors. We developed a rapid immunohistochemistry (R-IHC) method
using an alternating current electric field, which enables the completion of IHC in 20 min. We
performed intraoperative R-IHC on 16 glioma patients (9 with grade I-III gliomas and 7 with glioblastomas)
using the anti-mutant isocitrate dehydrogenase 1 (IDH1) monoclonal antibody (IDH1-
R132H ; H09), and the results were validated on permanent formalin-fixed paraffin-embedded
samples of the same patients by standard IHC. DNA sequencing of the IDH1 gene was also performed
for the 16 patients. Of 6 gliomas, 5 were shown to be positive by both R-IHC and standard
IHC, and IDH1 mutation was confirmed by DNA sequencing. According to the results of
R-IHC compared with both standard IHC results and DNA sequencing, the sensitivity of IDH1
R-IHC was 83% (5/6) ; specificity, 70% (7/10) ; positive predictive value, 63% (5/8) ; negative
predictive value, 88% (7/8) ; and accuracy, 75% (12/16). Thus, R-IHC using the anti-mutant
IDH1 monoclonal antibody has potential applications in the intraoperative identification of IDH1
mutations in gliomas.},
 pages = {147--156},
 title = {RAPID IMMUNOHISTOCHEMISTRY OF IDH-1 FOR THE INTRAOPERATIVE DIAGNOSIS OF GLIOMAS},
 volume = {42},
 year = {2016}
}