{"created":"2023-07-25T10:23:09.431405+00:00","id":2775,"links":{},"metadata":{"_buckets":{"deposit":"e7b1ae1b-bbc3-4686-ac27-2d4c29ccc5e3"},"_deposit":{"created_by":3,"id":"2775","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2775"},"status":"published"},"_oai":{"id":"oai:air.repo.nii.ac.jp:00002775","sets":["611:862:863:1089"]},"author_link":["10201","10198","10191","10196","10195","10193","10192","10202","10199","10190","10197","10200","10194"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3/4","bibliographicPageEnd":"156","bibliographicPageStart":"147","bibliographicVolumeNumber":"42","bibliographic_titles":[{"bibliographic_title":"秋田医学"}]}]},"item_10001_description_5":{"attribute_name":"内容記述（抄録）","attribute_value_mlt":[{"subitem_description":"Immunohistochemistry (IHC) plays a major role in the histopathological diagnosis of central nervous\nsystem (CNS) tumors. We developed a rapid immunohistochemistry (R-IHC) method\nusing an alternating current electric field, which enables the completion of IHC in 20 min. We\nperformed intraoperative R-IHC on 16 glioma patients (9 with grade I-III gliomas and 7 with glioblastomas)\nusing the anti-mutant isocitrate dehydrogenase 1 (IDH1) monoclonal antibody (IDH1-\nR132H ; H09), and the results were validated on permanent formalin-fixed paraffin-embedded\nsamples of the same patients by standard IHC. DNA sequencing of the IDH1 gene was also performed\nfor the 16 patients. Of 6 gliomas, 5 were shown to be positive by both R-IHC and standard\nIHC, and IDH1 mutation was confirmed by DNA sequencing. According to the results of\nR-IHC compared with both standard IHC results and DNA sequencing, the sensitivity of IDH1\nR-IHC was 83% (5/6) ; specificity, 70% (7/10) ; positive predictive value, 63% (5/8) ; negative\npredictive value, 88% (7/8) ; and accuracy, 75% (12/16). Thus, R-IHC using the anti-mutant\nIDH1 monoclonal antibody has potential applications in the intraoperative identification of IDH1\nmutations in gliomas.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"秋田医学会"}]},"item_10001_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AN00009294","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03866106","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"HIROSHIMA, Yuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"NANJO, Hiroshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SASAJIMA, Toshio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SHIMIZU, Hiroaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MINAMIYA, Yoshihiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YOSHIOKA, Toshiaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ODA, Masaya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KUDO-ASABE, Yukitsugu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TSUDA, Masumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TANINO, Mishie"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TANAKA, Shinya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"AKAGAMI, Yoichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"GOTO, Akiteru"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-16"}],"displaytype":"detail","filename":"akitai42-3_4(147).pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"akitai42-3_4(147).pdf","url":"https://air.repo.nii.ac.jp/record/2775/files/akitai42-3_4(147).pdf"},"version_id":"b93ef1a6-60d5-4a7a-93e5-322d91e43eb6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"IDH-1","subitem_subject_scheme":"Other"},{"subitem_subject":"rapid immunohistochemistry","subitem_subject_scheme":"Other"},{"subitem_subject":"glioma","subitem_subject_scheme":"Other"},{"subitem_subject":"intraoperative diagnosis","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA sequencing","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"RAPID IMMUNOHISTOCHEMISTRY OF IDH-1 FOR THE INTRAOPERATIVE DIAGNOSIS OF GLIOMAS","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"RAPID IMMUNOHISTOCHEMISTRY OF IDH-1 FOR THE INTRAOPERATIVE DIAGNOSIS OF GLIOMAS"}]},"item_type_id":"10001","owner":"3","path":["1089"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-08-01"},"publish_date":"2016-08-01","publish_status":"0","recid":"2775","relation_version_is_last":true,"title":["RAPID IMMUNOHISTOCHEMISTRY OF IDH-1 FOR THE INTRAOPERATIVE DIAGNOSIS OF GLIOMAS"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-25T11:32:07.992054+00:00"}