| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2010-02-09 |
| タイトル |
|
|
タイトル |
EXPRESSION OF XANTHINE OXIDASE IN TESTICULAR CELLS |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
xanthine oxidase |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
male infertility |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
cryptorchidism |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
xanthine dehydrogenase |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
testis |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 作成者 |
Kawaguchi, Satoshi
Fukuda, Jun
Kumagai, Jin
Shimizu, Yasushi
Kawamura, Kazuhiro
Tanaka, Toshinobu
|
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Objective : Previous studies showed that xanthine oxidase-related active oxygen generation was involved in heat stress-induced apoptosis in testicular cells. Hence, in the present study, the expression of xanthine oxidase in experimental cryptorchidism and heat-stressed testicular cells was assessed to determine the involvement of xanthine oxidase-related active oxygen generation in heat stress-induced apoptosis in testicular cells. Methods : (1) Immunohistological examination of xanthine oxidase was performed in a rat model of cryptorchidism. (2) mRNA expression of xanthine oxidase in cultured rat testicular cells was assessed by RT-PCR. (3) Expression of xanthine oxidase protein in cultured rat testicular cells was assessed by western blot analysis. Results : The presence of xanthine oxidase in testicular cells was confirmed by immunohistochemistry, especially after the spermatid stage. mRNA expression of xanthine oxidase in cultured testicular cells was similar with and without heat stress as determined by semiquantitative RTPCR. Expression of xanthine oxidase protein in cultured testicular cells was similar under both conditions, i.e., at 32.5˚C and at 37.0˚C, as determined by western blot analysis. Conclusions : mRNA and protein expression of xanthine oxidase was confirmed in testicular cells. Quantitative changes in xanthine oxidase expression cause by heat stress have not been clarified. These findings suggest that activate oxygen generated by xanthine oxidase is involved in apoptosis of testicular cells induced by heat stress. |
|
言語 |
en |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 書誌情報 |
ja : 秋田医学
巻 36,
号 2,
p. 99-105,
発行日 2009-11-01
|
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
03866106 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00009294 |
| 出版者 |
|
|
出版者 |
秋田医学会 |
|
言語 |
ja |
p99-105.pdf (2.1 MB)
