{"created":"2023-07-25T10:22:19.019113+00:00","id":1645,"links":{},"metadata":{"_buckets":{"deposit":"f333d6a9-e644-48d1-9c2e-37dbdb104109"},"_deposit":{"created_by":3,"id":"1645","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"1645"},"status":"published"},"_oai":{"id":"oai:air.repo.nii.ac.jp:00001645","sets":["611:862:863:877"]},"author_link":["5966","5968","5965","5967","5964","5969"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-11-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"105","bibliographicPageStart":"99","bibliographicVolumeNumber":"36","bibliographic_titles":[{"bibliographic_title":"秋田医学"}]}]},"item_10001_description_5":{"attribute_name":"内容記述（抄録）","attribute_value_mlt":[{"subitem_description":"Objective : Previous studies showed that xanthine oxidase-related active oxygen generation was involved in heat stress-induced apoptosis in testicular cells. Hence, in the present study, the expression\nof xanthine oxidase in experimental cryptorchidism and heat-stressed testicular cells was assessed to determine the involvement of xanthine oxidase-related active oxygen generation in heat stress-induced apoptosis in testicular cells. Methods : (1) Immunohistological examination\nof xanthine oxidase was performed in a rat model of cryptorchidism. (2) mRNA expression of xanthine oxidase in cultured rat testicular cells was assessed by RT-PCR. (3) Expression of xanthine oxidase protein in cultured rat testicular cells was assessed by western blot analysis.\nResults : The presence of xanthine oxidase in testicular cells was confirmed by immunohistochemistry, especially after the spermatid stage. mRNA expression of xanthine oxidase in cultured testicular cells was similar with and without heat stress as determined by semiquantitative RTPCR.\n Expression of xanthine oxidase protein in cultured testicular cells was similar under both conditions, i.e., at 32.5˚C and at 37.0˚C, as determined by western blot analysis. Conclusions : mRNA and protein expression of xanthine oxidase was confirmed in testicular cells. Quantitative changes in xanthine oxidase expression cause by heat stress have not been clarified. These findings suggest that activate oxygen generated by xanthine oxidase is involved in apoptosis of testicular cells induced by heat stress.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"秋田医学会"}]},"item_10001_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AN00009294","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03866106","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kawaguchi, Satoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukuda, Jun"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kumagai, Jin"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimizu, Yasushi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawamura, Kazuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Toshinobu"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-16"}],"displaytype":"detail","filename":"p99-105.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"p99-105.pdf","url":"https://air.repo.nii.ac.jp/record/1645/files/p99-105.pdf"},"version_id":"7faaeb89-3ba7-40be-bc1b-19196606c069"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"xanthine oxidase","subitem_subject_scheme":"Other"},{"subitem_subject":"male infertility","subitem_subject_scheme":"Other"},{"subitem_subject":"cryptorchidism","subitem_subject_scheme":"Other"},{"subitem_subject":"xanthine dehydrogenase","subitem_subject_scheme":"Other"},{"subitem_subject":"testis","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"EXPRESSION OF XANTHINE OXIDASE IN TESTICULAR CELLS","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"EXPRESSION OF XANTHINE OXIDASE IN TESTICULAR CELLS"}]},"item_type_id":"10001","owner":"3","path":["877"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-02-09"},"publish_date":"2010-02-09","publish_status":"0","recid":"1645","relation_version_is_last":true,"title":["EXPRESSION OF XANTHINE OXIDASE IN TESTICULAR CELLS"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-25T11:48:26.498986+00:00"}