@article{oai:air.repo.nii.ac.jp:00001645,
 author = {Kawaguchi, Satoshi and Fukuda, Jun and Kumagai, Jin and Shimizu, Yasushi and Kawamura, Kazuhiro and Tanaka, Toshinobu},
 issue = {2},
 journal = {秋田医学},
 month = {Nov},
 note = {Objective : Previous studies showed that xanthine oxidase-related active oxygen generation was involved in heat stress-induced apoptosis in testicular cells. Hence, in the present study, the expression
of xanthine oxidase in experimental cryptorchidism and heat-stressed testicular cells was assessed to determine the involvement of xanthine oxidase-related active oxygen generation in heat stress-induced apoptosis in testicular cells. Methods : (1) Immunohistological examination
of xanthine oxidase was performed in a rat model of cryptorchidism. (2) mRNA expression of xanthine oxidase in cultured rat testicular cells was assessed by RT-PCR. (3) Expression of xanthine oxidase protein in cultured rat testicular cells was assessed by western blot analysis.
Results : The presence of xanthine oxidase in testicular cells was confirmed by immunohistochemistry, especially after the spermatid stage. mRNA expression of xanthine oxidase in cultured testicular cells was similar with and without heat stress as determined by semiquantitative RTPCR.
 Expression of xanthine oxidase protein in cultured testicular cells was similar under both conditions, i.e., at 32.5˚C and at 37.0˚C, as determined by western blot analysis. Conclusions : mRNA and protein expression of xanthine oxidase was confirmed in testicular cells. Quantitative changes in xanthine oxidase expression cause by heat stress have not been clarified. These findings suggest that activate oxygen generated by xanthine oxidase is involved in apoptosis of testicular cells induced by heat stress.},
 pages = {99--105},
 title = {EXPRESSION OF XANTHINE OXIDASE IN TESTICULAR CELLS},
 volume = {36},
 year = {2009}
}