| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2009-11-30 |
| タイトル |
|
|
タイトル |
LIVE IMAGING BY TIME-LAPSE MICROSCOPY CAN MORE CLEARLY EVALUATE THE ANTI-APOPTOTIC STATE OF PRIMARY HEPATOCYTES ISOLATED FROM THE DRS KNOCKOUT MOUSE |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
time-lapse imaging |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
apoptosis |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
drs knockout mouse |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 作成者 |
FUKUMOTO, Kazuhiko
BO, Wang
HIKOSAKA, Keisuke
NORITAKE, Hidenao
SHIBATA, Kiyoshi
INOUE, Hirokazu
SUZUKI, Atsushi
INABA, Keisuke
SAKAGUCHI, Takanori
SUZUKI, Shohachi
KONNO, Hiroyuki
MIURA, Naoyuki
|
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
To investigate the anti-apoptotic state of primary hepatocytes isolated from the drs knockout(KO)mice, Fas-mediated apoptosis was observed by flow cytometry and time-lapse microscopy. Treatment with anti-Fas antibody plus actinomycin D or cycloheximide induced over 50% apoptosis for primary hepatocytes based on annexin V staining. At 24 hr later under these apoptotic stimuli, the sub G1 fraction of hepatocytes isolated from the drs KO mice was significantly decreased in comparison to wild C57BL/ 6 mice. In contrast, sequential time-lapse microscopic analysis revealed clearer and larger differences in the apoptotic responses of primary hepatocytes from the drs KO and wild-type mice from 4 hr to 24 hr after apoptotic induction. The time-lapse microscopic analysis clearly indicated the hepatocytes isolated from the drs KO mouse to be more resistant to the apoptotic stimuli than other methods. The time-lapse microscopy is very useful for the sequential observation of viable primary hepatocytes. |
|
言語 |
en |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 書誌情報 |
ja : 秋田医学
巻 35,
号 3/4,
p. 231-237,
発行日 2009-03-01
|
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
03866106 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00009294 |
| 出版者 |
|
|
出版者 |
秋田医学会 |
|
言語 |
ja |
p231-237.pdf (568.1 kB)
