WEKO3
アイテム
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LIVE IMAGING BY TIME-LAPSE MICROSCOPY CAN MORE CLEARLY EVALUATE THE ANTI-APOPTOTIC STATE OF PRIMARY HEPATOCYTES ISOLATED FROM THE DRS KNOCKOUT MOUSE
http://hdl.handle.net/10295/1624
http://hdl.handle.net/10295/1624c9be6f44-a5d9-4638-adb1-c62862b19198
名前 / ファイル | ライセンス | アクション |
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p231-237.pdf (568.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-11-30 | |||||
タイトル | ||||||
タイトル | LIVE IMAGING BY TIME-LAPSE MICROSCOPY CAN MORE CLEARLY EVALUATE THE ANTI-APOPTOTIC STATE OF PRIMARY HEPATOCYTES ISOLATED FROM THE DRS KNOCKOUT MOUSE | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | time-lapse imaging | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | apoptosis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | drs knockout mouse | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
FUKUMOTO, Kazuhiko
× FUKUMOTO, Kazuhiko× BO, Wang× HIKOSAKA, Keisuke× NORITAKE, Hidenao× SHIBATA, Kiyoshi× INOUE, Hirokazu× SUZUKI, Atsushi× INABA, Keisuke× SAKAGUCHI, Takanori× SUZUKI, Shohachi× KONNO, Hiroyuki× MIURA, Naoyuki |
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内容記述(抄録) | ||||||
内容記述タイプ | Other | |||||
内容記述 | To investigate the anti-apoptotic state of primary hepatocytes isolated from the drs knockout(KO)mice, Fas-mediated apoptosis was observed by flow cytometry and time-lapse microscopy. Treatment with anti-Fas antibody plus actinomycin D or cycloheximide induced over 50% apoptosis for primary hepatocytes based on annexin V staining. At 24 hr later under these apoptotic stimuli, the sub G1 fraction of hepatocytes isolated from the drs KO mice was significantly decreased in comparison to wild C57BL/ 6 mice. In contrast, sequential time-lapse microscopic analysis revealed clearer and larger differences in the apoptotic responses of primary hepatocytes from the drs KO and wild-type mice from 4 hr to 24 hr after apoptotic induction. The time-lapse microscopic analysis clearly indicated the hepatocytes isolated from the drs KO mouse to be more resistant to the apoptotic stimuli than other methods. The time-lapse microscopy is very useful for the sequential observation of viable primary hepatocytes. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
書誌情報 |
秋田医学 巻 35, 号 3/4, p. 231-237, 発行日 2009-03-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03866106 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00009294 | |||||
出版者 | ||||||
出版者 | 秋田医学会 |