{"created":"2023-07-25T10:22:17.650168+00:00","id":1614,"links":{},"metadata":{"_buckets":{"deposit":"2e6a6d22-03aa-4814-bf29-5ac9f4b0898b"},"_deposit":{"created_by":3,"id":"1614","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"1614"},"status":"published"},"_oai":{"id":"oai:air.repo.nii.ac.jp:00001614","sets":["611:862:863:872"]},"author_link":["5835","5840","5838","5839","5837","5841","5842","5843","5844","5845","5846","5836"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3/4","bibliographicPageEnd":"237","bibliographicPageStart":"231","bibliographicVolumeNumber":"35","bibliographic_titles":[{"bibliographic_title":"秋田医学"}]}]},"item_10001_description_5":{"attribute_name":"内容記述（抄録）","attribute_value_mlt":[{"subitem_description":"To investigate the anti-apoptotic state of primary hepatocytes isolated from the drs knockout(KO)mice, Fas-mediated apoptosis was observed by flow cytometry and time-lapse microscopy. Treatment with anti-Fas antibody plus actinomycin D or cycloheximide induced over 50% apoptosis for primary hepatocytes based on annexin V staining. At 24 hr later under these apoptotic stimuli, the sub G1 fraction of hepatocytes isolated from the drs KO mice was significantly decreased in comparison to wild C57BL/ 6 mice. In contrast, sequential time-lapse microscopic analysis revealed clearer and larger differences in the apoptotic responses of primary hepatocytes from the drs KO and wild-type mice from 4 hr to 24 hr after apoptotic induction. The time-lapse microscopic analysis clearly indicated the hepatocytes isolated from the drs KO mouse to be more resistant to the apoptotic stimuli than other methods. The time-lapse microscopy is very useful for the sequential observation of viable primary hepatocytes.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"秋田医学会"}]},"item_10001_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AN00009294","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03866106","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"FUKUMOTO, Kazuhiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"BO, Wang"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HIKOSAKA, Keisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"NORITAKE, Hidenao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SHIBATA, Kiyoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"INOUE, Hirokazu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SUZUKI, Atsushi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"INABA, Keisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SAKAGUCHI, Takanori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SUZUKI, Shohachi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KONNO, Hiroyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MIURA, Naoyuki"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-16"}],"displaytype":"detail","filename":"p231-237.pdf","filesize":[{"value":"568.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"p231-237.pdf","url":"https://air.repo.nii.ac.jp/record/1614/files/p231-237.pdf"},"version_id":"85ca6a9b-58d4-45dd-8dbd-dc4df5bec6e4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"time-lapse imaging","subitem_subject_scheme":"Other"},{"subitem_subject":"apoptosis","subitem_subject_scheme":"Other"},{"subitem_subject":"drs knockout mouse","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"LIVE IMAGING BY TIME-LAPSE MICROSCOPY CAN MORE CLEARLY EVALUATE THE ANTI-APOPTOTIC STATE OF PRIMARY HEPATOCYTES ISOLATED FROM THE DRS KNOCKOUT MOUSE","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"LIVE IMAGING BY TIME-LAPSE MICROSCOPY CAN MORE CLEARLY EVALUATE THE ANTI-APOPTOTIC STATE OF PRIMARY HEPATOCYTES ISOLATED FROM THE DRS KNOCKOUT MOUSE"}]},"item_type_id":"10001","owner":"3","path":["872"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-11-30"},"publish_date":"2009-11-30","publish_status":"0","recid":"1614","relation_version_is_last":true,"title":["LIVE IMAGING BY TIME-LAPSE MICROSCOPY CAN MORE CLEARLY EVALUATE THE ANTI-APOPTOTIC STATE OF PRIMARY HEPATOCYTES ISOLATED FROM THE DRS KNOCKOUT MOUSE"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-25T11:48:50.918240+00:00"}