@article{oai:air.repo.nii.ac.jp:00001614,
 author = {FUKUMOTO, Kazuhiko and BO, Wang and HIKOSAKA, Keisuke and NORITAKE, Hidenao and SHIBATA, Kiyoshi and INOUE, Hirokazu and SUZUKI, Atsushi and INABA, Keisuke and SAKAGUCHI, Takanori and SUZUKI, Shohachi and KONNO, Hiroyuki and MIURA, Naoyuki},
 issue = {3/4},
 journal = {秋田医学},
 month = {Mar},
 note = {To investigate the anti-apoptotic state of primary hepatocytes isolated from the drs knockout(KO)mice, Fas-mediated apoptosis was observed by flow cytometry and time-lapse microscopy. Treatment with anti-Fas antibody plus actinomycin D or cycloheximide induced over 50% apoptosis for primary hepatocytes based on annexin V staining. At 24 hr later under these apoptotic stimuli, the sub G1 fraction of hepatocytes isolated from the drs KO mice was significantly decreased in comparison to wild C57BL/ 6 mice. In contrast, sequential time-lapse microscopic analysis revealed clearer and larger differences in the apoptotic responses of primary hepatocytes from the drs KO and wild-type mice from 4 hr to 24 hr after apoptotic induction. The time-lapse microscopic analysis clearly indicated the hepatocytes isolated from the drs KO mouse to be more resistant to the apoptotic stimuli than other methods. The time-lapse microscopy is very useful for the sequential observation of viable primary hepatocytes.},
 pages = {231--237},
 title = {LIVE IMAGING BY TIME-LAPSE MICROSCOPY CAN MORE CLEARLY EVALUATE THE ANTI-APOPTOTIC STATE OF PRIMARY HEPATOCYTES ISOLATED FROM THE DRS KNOCKOUT MOUSE},
 volume = {35},
 year = {2009}
}