| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2023-02-25 |
| タイトル |
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タイトル |
Production of the medaka derived from vitrified whole testes by germ cell transplantation |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 作成者 |
Seki, Shinsuke
Kusano, Kazunari
Lee, Seungki
Iwasaki, Yoshiko
Yagisawa, Masaru
Ishida, Mariko
Hiratsuka, Tadashi
Sasado, Takao
Naruse, Kiyoshi
Yoshizaki, Goro
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| 内容記述 |
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内容記述タイプ |
Abstract |
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内容記述 |
The medaka (Oryzias latipes) is a teleost model distinguished from other model organisms by the presence of inbred strains, wild stocks, and related species. Cryopreservation guarantees preservation of these unique biological resources. However, because of their large size, cryopreservation techniques for their eggs and embryos have not been established. In the present study, we established a methodology to produce functional gametes from cryopreserved testicular cells (TCs). Whole testes taken from medaka were cryopreserved by vitrification. After thawing, the cells dissociated from cryopreserved testicular tissues were intraperitoneally transplanted into sterile triploid hatchlings. Some cells, presumably spermatogonial stem cells, migrated into the genital ridges of recipients and resulted in the production of eggs or sperm, based on sex of the recipient. Mating of recipients resulted in successful production of cryopreserved TC-derived offspring. We successfully produced individuals from the Kaga inbred line, an endangered wild population in Tokyo, and a sub-fertile mutant (wnt4b(-/-)) from cryopreserved their TCs. This methodology facilitates semi-permanent preservation of various medaka strains. |
|
言語 |
en |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 書誌情報 |
en : SCIENTIFIC REPORTS
巻 7,
発行日 2017
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| 収録物識別子 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
2045-2322 |
| 出版者 |
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出版者 |
Nature Research |
|
言語 |
en |
| 関連情報 |
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関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1038/srep43185 |
| 権利情報 |
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権利情報 |
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ © The Author(s) 2017 |
bioA_2022_1.pdf (5.7 MB)
