{"created":"2023-07-25T10:25:34.634735+00:00","id":5977,"links":{},"metadata":{"_buckets":{"deposit":"95170dcc-07a9-43fb-a22e-89d8336e4ff1"},"_deposit":{"created_by":15,"id":"5977","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"5977"},"status":"published"},"_oai":{"id":"oai:air.repo.nii.ac.jp:00005977","sets":["1496:1497:1498"]},"author_link":[],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"7","bibliographic_titles":[{"bibliographic_title":"SCIENTIFIC REPORTS","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"The medaka (Oryzias latipes) is a teleost model distinguished from other model organisms by the presence of inbred strains, wild stocks, and related species. Cryopreservation guarantees preservation of these unique biological resources. However, because of their large size, cryopreservation techniques for their eggs and embryos have not been established. In the present study, we established a methodology to produce functional gametes from cryopreserved testicular cells (TCs). Whole testes taken from medaka were cryopreserved by vitrification. After thawing, the cells dissociated from cryopreserved testicular tissues were intraperitoneally transplanted into sterile triploid hatchlings. Some cells, presumably spermatogonial stem cells, migrated into the genital ridges of recipients and resulted in the production of eggs or sperm, based on sex of the recipient. Mating of recipients resulted in successful production of cryopreserved TC-derived offspring. We successfully produced individuals from the Kaga inbred line, an endangered wild population in Tokyo, and a sub-fertile mutant (wnt4b(-/-)) from cryopreserved their TCs. This methodology facilitates semi-permanent preservation of various medaka strains.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature Research","subitem_publisher_language":"en"}]},"item_10001_relation_25":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/srep43185","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ © The Author(s) 2017"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1724099666130":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"2045-2322","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Seki, Shinsuke","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kusano, Kazunari","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Lee, Seungki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Iwasaki, Yoshiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yagisawa, Masaru","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ishida, Mariko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hiratsuka, Tadashi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Sasado, Takao","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Naruse, Kiyoshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yoshizaki, Goro"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-25"}],"displaytype":"detail","filename":"bioA_2022_1.pdf","filesize":[{"value":"5.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bioA_2022_1.pdf","url":"https://air.repo.nii.ac.jp/record/5977/files/bioA_2022_1.pdf"},"version_id":"8ddbd1e4-23a5-4da2-b94f-89e6b528c097"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Production of the medaka derived from vitrified whole testes by germ cell transplantation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Production of the medaka derived from vitrified whole testes by germ cell transplantation","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"15","path":["1498"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-02-25"},"publish_date":"2023-02-25","publish_status":"0","recid":"5977","relation_version_is_last":true,"title":["Production of the medaka derived from vitrified whole testes by germ cell transplantation"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-08-22T23:15:55.144569+00:00"}