| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2014-06-16 |
| タイトル |
|
|
タイトル |
Characterization of Glycosylphosphatidylinositol-Anchored Ceruloplasmin Enriched in the Apical Plasma Membrane of Rat Hepatocytes |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
apical membrane |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
ceruloplasmin |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
GPI-Cp |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
hepatocyte |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 作成者 |
Flores, Maria Jolina Lou N.
Koyota, Souichi
Qiao, Zhiwei
Koizumi, Yukio
Sugiyama, Toshihiro
|
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Aim : Ceruloplasmin (Cp) is an acute-phase protein and a member of the multicopper oxidase family of enzymes. It has been implicated in iron metabolism because of its ferroxidase activity. It is expressed as soluble (sCp) or glycosylphosphatidylinositol-anchored ceruloplasmin (GPI-Cp) form ; the former is primarily synthesized in the liver, and the latter is primarily found in the brain. Although recent studies reported GPI-Cp expression on hepatocytes, little is known regarding its presence in specific liver cell compartments and its possible involvement in liver pathophysiology. This study aimed to characterize the distribution of GPI-Cp in liver cells and specifically in the apical part of the plasma membrane. Methods : We assessed GPI-Cp expression in the liver using immunohistochemistry and immunoblotting techniques. Furthermore, we isolated apical and basolateral membrane fraction from the total liver membrane using sucrose discontinuous gradient centrifugation, and GPI-Cp were detected using immunoblotting. Results : GPI-Cp was detected in purified apical membranes of rat liver cells. Immunoreactive Cp protein was released after incubation with phosphatidylinositol-specific phospholipase C, and the free protein demonstrated ferroxidase activity. Conclusion : These findings suggest that majority of GPI-Cp present in the liver is primarily located on the apical surface of cells because of transcytosis. |
|
言語 |
en |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 書誌情報 |
ja : 秋田医学
巻 40,
号 3/4,
p. 163-173,
発行日 2014-03-31
|
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
03866106 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00009294 |
| 出版者 |
|
|
出版者 |
秋田医学会 |
|
言語 |
ja |
akitai40-3_4(163).pdf (1.6 MB)
