{"created":"2023-07-25T10:22:48.548520+00:00","id":2298,"links":{},"metadata":{"_buckets":{"deposit":"fcd83537-da15-4895-8e91-00b293f8823b"},"_deposit":{"created_by":3,"id":"2298","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2298"},"status":"published"},"_oai":{"id":"oai:air.repo.nii.ac.jp:00002298","sets":["611:862:863:1003"]},"author_link":["8635","8634","8633","8632","8631"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3/4","bibliographicPageEnd":"173","bibliographicPageStart":"163","bibliographicVolumeNumber":"40","bibliographic_titles":[{"bibliographic_title":"秋田医学"}]}]},"item_10001_description_5":{"attribute_name":"内容記述（抄録）","attribute_value_mlt":[{"subitem_description":"Aim : Ceruloplasmin (Cp) is an acute-phase protein and a member of the multicopper oxidase family of enzymes. It has been implicated in iron metabolism because of its ferroxidase activity. It is expressed as soluble (sCp) or glycosylphosphatidylinositol-anchored ceruloplasmin (GPI-Cp) form ; the former is primarily synthesized in the liver, and the latter is primarily found in the brain. Although recent studies reported GPI-Cp expression on hepatocytes, little is known regarding its presence in specific liver cell compartments and its possible involvement in liver pathophysiology. This study aimed to characterize the distribution of GPI-Cp in liver cells and specifically in the apical part of the plasma membrane. Methods : We assessed GPI-Cp expression in the liver using immunohistochemistry and immunoblotting techniques. Furthermore, we isolated apical and basolateral membrane fraction from the total liver membrane using sucrose discontinuous gradient centrifugation, and GPI-Cp were detected using immunoblotting. Results : GPI-Cp was detected in purified apical membranes of rat liver cells. Immunoreactive Cp protein was released after incubation with phosphatidylinositol-specific phospholipase C, and the free protein demonstrated ferroxidase activity. Conclusion : These findings suggest that majority of GPI-Cp present in the liver is primarily located on the apical surface of cells because of transcytosis.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"秋田医学"}]},"item_10001_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AN00009294","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03866106","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Flores, Maria Jolina Lou N."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Koyota, Souichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Qiao, Zhiwei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Koizumi, Yukio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sugiyama, Toshihiro"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-16"}],"displaytype":"detail","filename":"akitai40-3_4(163).pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"akitai40-3_4(163).pdf","url":"https://air.repo.nii.ac.jp/record/2298/files/akitai40-3_4(163).pdf"},"version_id":"4e8b1c06-0236-4fdb-a4cf-6e5f63d6887d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"apical membrane","subitem_subject_scheme":"Other"},{"subitem_subject":"ceruloplasmin","subitem_subject_scheme":"Other"},{"subitem_subject":"GPI-Cp","subitem_subject_scheme":"Other"},{"subitem_subject":"hepatocyte","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of Glycosylphosphatidylinositol-Anchored Ceruloplasmin Enriched in the Apical Plasma Membrane of Rat Hepatocytes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of Glycosylphosphatidylinositol-Anchored Ceruloplasmin Enriched in the Apical Plasma Membrane of Rat Hepatocytes"}]},"item_type_id":"10001","owner":"3","path":["1003"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-06-16"},"publish_date":"2014-06-16","publish_status":"0","recid":"2298","relation_version_is_last":true,"title":["Characterization of Glycosylphosphatidylinositol-Anchored Ceruloplasmin Enriched in the Apical Plasma Membrane of Rat Hepatocytes"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-25T11:33:00.787911+00:00"}