{"_buckets": {"deposit": "55ff10f1-8781-45b5-8e95-7ada24b229ab"}, "_deposit": {"created_by": 3, "id": "2296", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "2296"}, "status": "published"}, "_oai": {"id": "oai:air.repo.nii.ac.jp:00002296", "sets": ["970"]}, "author_link": ["8626", "8625"], "item_10006_alternative_title_32": {"attribute_name": "別タイトル", "attribute_value_mlt": [{"subitem_alternative_title": "ラット肝細胞の頂端面側細胞膜に局在するGPI-セルロプラスミンの特性に関する研究"}]}, "item_10006_biblio_info_34": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2014-06-01", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_10006_date_granted_41": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2014-03-22"}]}, "item_10006_degree_grantor_40": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "秋田大学"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "11401", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_10006_degree_name_39": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（医学）"}]}, "item_10006_description_27": {"attribute_name": "内容記述（抄録）", "attribute_value_mlt": [{"subitem_description": "Aim: Ceruloplasmin (Cp) is an acute-phase protein and a member of the multicopper oxidase family of enzymes. It has been implicated in iron metabolism because of its ferroxidase activity. It is expressed as soluble (sCp) or glycosylphosphatidylinositolanchored ceruloplasmin (GPI-Cp) form; the former is primarily synthesized in the liver, and the latter is primarily found in the brain. Although recent studies reported GPI-Cp expression on hepatocytes, little is known regarding its presence in specific liver cell compartments and its possible involvement in liver pathophysiology. This study aimed to characterize the distribution of GPI-Cp in liver cells and specifically in the apical part of the plasma membrane. Methods: We assessed GPI-Cp expression in the liver using immunohistochemistry and immunoblotting techniques. Furthermore, we isolated apical and basolateral membrane fraction from the total liver membrane using sucrose discontinuous gradient centrifugation, and GPI-Cp were detected using immunoblotting. Results: GPI-Cp was detected in purified apical membranes of rat liver cells. Immunoreactive Cp protein was released after incubation with phosphatidylinositolspecific phospholipase C, and the free protein demonstrated ferroxidase activity. Conclusion: These findings suggest that majority of GPI-Cp present in the liver is primarily located on the apical surface of cells because of transcytosis.", "subitem_description_type": "Other"}]}, "item_10006_dissertation_number_42": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "甲第1088号"}]}, "item_10006_relation_30": {"attribute_name": "関連リンク", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "http://hdl.handle.net/10295/2507"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://hdl.handle.net/10295/2507", "subitem_relation_type_select": "HDL"}}]}, "item_10006_text_36": {"attribute_name": "備考", "attribute_value_mlt": [{"subitem_text_value": "秋田大学審査学位論文（秋田医学,40(3/4),p163-p173掲載論文の著者最終稿）"}]}, "item_10006_version_type_35": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "フローレス, マリアジョリナルー"}], "nameIdentifiers": [{"nameIdentifier": "8625", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Flores, Maria Jolina Lou", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8626", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-02-16"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ihakukou1088.pdf", "filesize": [{"value": "2.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2300000.0, "url": {"label": "本文", "url": "https://air.repo.nii.ac.jp/record/2296/files/ihakukou1088.pdf"}, "version_id": "415663e5-8cd5-4f26-997b-3ba51a6c3737"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "apical membrane", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ceruloplasmin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "GPI-Cp", "subitem_subject_scheme": "Other"}, {"subitem_subject": "hepatocyte", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "Characterization of glycosylphosphatidylinositol-anchored ceruloplasmin enriched in the apical plasma membrane of rat hepatocytes", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Characterization of glycosylphosphatidylinositol-anchored ceruloplasmin enriched in the apical plasma membrane of rat hepatocytes"}]}, "item_type_id": "10006", "owner": "3", "path": ["970"], "permalink_uri": "http://hdl.handle.net/10295/2509", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-06-23"}, "publish_date": "2014-06-23", "publish_status": "0", "recid": "2296", "relation": {}, "relation_version_is_last": true, "title": ["Characterization of glycosylphosphatidylinositol-anchored ceruloplasmin enriched in the apical plasma membrane of rat hepatocytes"], "weko_shared_id": 3}