| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2014-06-23 |
| タイトル |
|
|
タイトル |
Characterization of glycosylphosphatidylinositol-anchored ceruloplasmin enriched in the apical plasma membrane of rat hepatocytes |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
apical membrane |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
ceruloplasmin |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
GPI-Cp |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
hepatocyte |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 別タイトル |
|
|
その他のタイトル |
ラット肝細胞の頂端面側細胞膜に局在するGPI-セルロプラスミンの特性に関する研究 |
| 作成者 |
フローレス, マリアジョリナルー
Flores, Maria Jolina Lou
|
| 内容記述（抄録） |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Aim: Ceruloplasmin (Cp) is an acute-phase protein and a member of the multicopper oxidase family of enzymes. It has been implicated in iron metabolism because of its ferroxidase activity. It is expressed as soluble (sCp) or glycosylphosphatidylinositolanchored ceruloplasmin (GPI-Cp) form; the former is primarily synthesized in the liver, and the latter is primarily found in the brain. Although recent studies reported GPI-Cp expression on hepatocytes, little is known regarding its presence in specific liver cell compartments and its possible involvement in liver pathophysiology. This study aimed to characterize the distribution of GPI-Cp in liver cells and specifically in the apical part of the plasma membrane. Methods: We assessed GPI-Cp expression in the liver using immunohistochemistry and immunoblotting techniques. Furthermore, we isolated apical and basolateral membrane fraction from the total liver membrane using sucrose discontinuous gradient centrifugation, and GPI-Cp were detected using immunoblotting. Results: GPI-Cp was detected in purified apical membranes of rat liver cells. Immunoreactive Cp protein was released after incubation with phosphatidylinositolspecific phospholipase C, and the free protein demonstrated ferroxidase activity. Conclusion: These findings suggest that majority of GPI-Cp present in the liver is primarily located on the apical surface of cells because of transcytosis. |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 書誌情報 |
発行日 2014-06-01
|
| 関連リンク |
|
|
|
識別子タイプ |
HDL |
|
|
関連識別子 |
http://hdl.handle.net/10295/2507 |
|
|
関連名称 |
http://hdl.handle.net/10295/2507 |
| 備考 |
|
|
|
秋田大学審査学位論文（秋田医学,40(3/4),p163-p173掲載論文の著者最終稿） |
| 学位名 |
|
|
学位名 |
博士（医学） |
| 学位授与機関 |
|
|
|
学位授与機関識別子Scheme |
kakenhi |
|
|
学位授与機関識別子 |
11401 |
|
|
学位授与機関名 |
秋田大学 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2014-03-22 |
| 学位授与番号 |
|
|
学位授与番号 |
甲第1088号 |
本文 (2.3 MB)
