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Characterization of glycosylphosphatidylinositol-anchored ceruloplasmin enriched in the apical plasma membrane of rat hepatocytes
http://hdl.handle.net/10295/2509
http://hdl.handle.net/10295/2509c4995437-d1b1-4867-b6a9-30a77bcb8aa0
名前 / ファイル | ライセンス | アクション |
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本文 (2.3 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2014-06-23 | |||||
タイトル | ||||||
タイトル | Characterization of glycosylphosphatidylinositol-anchored ceruloplasmin enriched in the apical plasma membrane of rat hepatocytes | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | apical membrane | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ceruloplasmin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | GPI-Cp | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | hepatocyte | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
別タイトル | ||||||
その他のタイトル | ラット肝細胞の頂端面側細胞膜に局在するGPI-セルロプラスミンの特性に関する研究 | |||||
作成者 |
フローレス, マリアジョリナルー
× フローレス, マリアジョリナルー× Flores, Maria Jolina Lou |
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内容記述(抄録) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Aim: Ceruloplasmin (Cp) is an acute-phase protein and a member of the multicopper oxidase family of enzymes. It has been implicated in iron metabolism because of its ferroxidase activity. It is expressed as soluble (sCp) or glycosylphosphatidylinositolanchored ceruloplasmin (GPI-Cp) form; the former is primarily synthesized in the liver, and the latter is primarily found in the brain. Although recent studies reported GPI-Cp expression on hepatocytes, little is known regarding its presence in specific liver cell compartments and its possible involvement in liver pathophysiology. This study aimed to characterize the distribution of GPI-Cp in liver cells and specifically in the apical part of the plasma membrane. Methods: We assessed GPI-Cp expression in the liver using immunohistochemistry and immunoblotting techniques. Furthermore, we isolated apical and basolateral membrane fraction from the total liver membrane using sucrose discontinuous gradient centrifugation, and GPI-Cp were detected using immunoblotting. Results: GPI-Cp was detected in purified apical membranes of rat liver cells. Immunoreactive Cp protein was released after incubation with phosphatidylinositolspecific phospholipase C, and the free protein demonstrated ferroxidase activity. Conclusion: These findings suggest that majority of GPI-Cp present in the liver is primarily located on the apical surface of cells because of transcytosis. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
書誌情報 | 発行日 2014-06-01 | |||||
関連リンク | ||||||
識別子タイプ | HDL | |||||
関連識別子 | http://hdl.handle.net/10295/2507 | |||||
関連名称 | http://hdl.handle.net/10295/2507 | |||||
備考 | ||||||
値 | 秋田大学審査学位論文(秋田医学,40(3/4),p163-p173掲載論文の著者最終稿) | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 11401 | |||||
学位授与機関名 | 秋田大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2014-03-22 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲第1088号 |