{"created":"2023-07-25T10:22:48.463258+00:00","id":2296,"links":{},"metadata":{"_buckets":{"deposit":"55ff10f1-8781-45b5-8e95-7ada24b229ab"},"_deposit":{"created_by":3,"id":"2296","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2296"},"status":"published"},"_oai":{"id":"oai:air.repo.nii.ac.jp:00002296","sets":["611:968:969:970"]},"author_link":["8626","8625"],"item_10006_alternative_title_32":{"attribute_name":"別タイトル","attribute_value_mlt":[{"subitem_alternative_title":"ラット肝細胞の頂端面側細胞膜に局在するGPI-セルロプラスミンの特性に関する研究"}]},"item_10006_biblio_info_34":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-06-01","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{}]}]},"item_10006_date_granted_41":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2014-03-22"}]},"item_10006_degree_grantor_40":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"秋田大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"11401","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_39":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）"}]},"item_10006_description_27":{"attribute_name":"内容記述（抄録）","attribute_value_mlt":[{"subitem_description":"Aim: Ceruloplasmin (Cp) is an acute-phase protein and a member of the multicopper oxidase family of enzymes. It has been implicated in iron metabolism because of its ferroxidase activity. It is expressed as soluble (sCp) or glycosylphosphatidylinositolanchored ceruloplasmin (GPI-Cp) form; the former is primarily synthesized in the liver, and the latter is primarily found in the brain. Although recent studies reported GPI-Cp expression on hepatocytes, little is known regarding its presence in specific liver cell compartments and its possible involvement in liver pathophysiology. This study aimed to characterize the distribution of GPI-Cp in liver cells and specifically in the apical part of the plasma membrane. Methods: We assessed GPI-Cp expression in the liver using immunohistochemistry and immunoblotting techniques. Furthermore, we isolated apical and basolateral membrane fraction from the total liver membrane using sucrose discontinuous gradient centrifugation, and GPI-Cp were detected using immunoblotting. Results: GPI-Cp was detected in purified apical membranes of rat liver cells. Immunoreactive Cp protein was released after incubation with phosphatidylinositolspecific phospholipase C, and the free protein demonstrated ferroxidase activity. Conclusion: These findings suggest that majority of GPI-Cp present in the liver is primarily located on the apical surface of cells because of transcytosis.","subitem_description_type":"Other"}]},"item_10006_dissertation_number_42":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第1088号"}]},"item_10006_relation_30":{"attribute_name":"関連リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://hdl.handle.net/10295/2507"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://hdl.handle.net/10295/2507","subitem_relation_type_select":"HDL"}}]},"item_10006_text_36":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_value":"秋田大学審査学位論文（秋田医学,40(3/4),p163-p173掲載論文の著者最終稿）"}]},"item_10006_version_type_35":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"フローレス, マリアジョリナルー"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Flores, Maria Jolina Lou","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-16"}],"displaytype":"detail","filename":"ihakukou1088.pdf","filesize":[{"value":"2.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","url":"https://air.repo.nii.ac.jp/record/2296/files/ihakukou1088.pdf"},"version_id":"415663e5-8cd5-4f26-997b-3ba51a6c3737"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"apical membrane","subitem_subject_scheme":"Other"},{"subitem_subject":"ceruloplasmin","subitem_subject_scheme":"Other"},{"subitem_subject":"GPI-Cp","subitem_subject_scheme":"Other"},{"subitem_subject":"hepatocyte","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Characterization of glycosylphosphatidylinositol-anchored ceruloplasmin enriched in the apical plasma membrane of rat hepatocytes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of glycosylphosphatidylinositol-anchored ceruloplasmin enriched in the apical plasma membrane of rat hepatocytes"}]},"item_type_id":"10006","owner":"3","path":["970"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-06-23"},"publish_date":"2014-06-23","publish_status":"0","recid":"2296","relation_version_is_last":true,"title":["Characterization of glycosylphosphatidylinositol-anchored ceruloplasmin enriched in the apical plasma membrane of rat hepatocytes"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-25T11:33:02.178038+00:00"}