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Intrinsic Oncogenic Function of Intracellular Connexin26 Protein in Head and Neck Squamous Cell Carcinoma Cells
http://hdl.handle.net/10295/00006202
http://hdl.handle.net/10295/00006202042d8fd9-dae0-4aea-9d20-089ff9420cdf
名前 / ファイル | ライセンス | アクション |
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iA_2022_59.pdf (2.7 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2023-02-23 | |||||
タイトル | ||||||
タイトル | Intrinsic Oncogenic Function of Intracellular Connexin26 Protein in Head and Neck Squamous Cell Carcinoma Cells | |||||
言語 | en | |||||
言語 | ||||||
言語 | eng | |||||
主題 | ||||||
主題Scheme | Other | |||||
主題 | gap junction | |||||
主題 | ||||||
主題Scheme | Other | |||||
主題 | cell-cell communication | |||||
主題 | ||||||
主題Scheme | Other | |||||
主題 | connexin26 | |||||
主題 | ||||||
主題Scheme | Other | |||||
主題 | head and neck squamous cell carcinoma | |||||
主題 | ||||||
主題Scheme | Other | |||||
主題 | ER-Golgi retention signal | |||||
主題 | ||||||
主題Scheme | Other | |||||
主題 | cancer progression | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
作成者 |
Iikawa, Nobuko
× Iikawa, Nobuko× Yamamoto, Yohei× Kawasaki, Yohei× Nishijima-Matsunobu, Aki× Suzuki, Maya× Yamada, Takechiyo× Omori, Yasufumi |
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内容記述 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | It has long been known that the gap junction is down-regulated in many tumours. One of the downregulation mechanisms is the translocation of connexin, a gap junction protein, from cell membrane into cytoplasm, nucleus, or Golgi apparatus. Interestingly, as tumours progress and reinforce their malignant phenotype, the amount of aberrantly-localised connexin increases in different malignant tumours including oesophageal squamous cell carcinoma, thus suggesting that such an aberrantly-localised connexin should be oncogenic, although gap junctional connexins are often tumour-suppressive. To define the dual roles of connexin in head and neck squamous cell carcinoma (HNSCC), we introduced the wild-type connexin26 (wtCx26) or the mutant Cx26 (icCx26) gene, the product of which carries the amino acid sequence AKKFF, an endoplasmic reticulum-Golgi retention signal, at the C-terminus and is not sorted to cell membrane, into the human FaDu hypopharyngeal cancer cell line that had severely impaired the expression of connexin during carcinogenesis. wtCx26 protein was trafficked to the cell membrane and formed gap junction, which successfully exerted cell-cell communication. On the other hand, the icCx26 protein was co-localised with a Golgi marker, as revealed by immunofluorescence, and thus was retained on the way to the cell membrane. While the forced expression of wtCx26 suppressed both cell proliferation in vitro and tumorigenicity in mice in vivo, icCx26 significantly enhanced both cell proliferation and tumorigenicity compared with the mock control clones, indicating that an excessive accumulation of connexin protein in intracellular domains should be involved in cancer progression and that restoration of proper subcellular sorting of connexin might be a therapeutic strategy to control HNSCC. | |||||
言語 | en | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
書誌情報 |
en : INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 巻 19, 号 7, 発行日 2018 |
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収録物識別子 | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1422-0067 | |||||
出版者 | ||||||
出版者 | MDPI | |||||
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関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.3390/ijms19072134 | |||||
権利情報 | ||||||
権利情報 | © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |