| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2021-08-10 |
| タイトル |
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|
タイトル |
Rapid HER2 cytologic fluorescence in situ hybridization for breast cancer using noncontact alternating current electric field mixing |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
breast cancercytology |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
human epidermal growth factor receptor 2 |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
in situ hybridization |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
noncontact alternating current electric field mixing |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 作成者 |
Watanabe, Shin-nosuke
Imai, Kazuhiro
Nanjo, Hiroshi
Wakamatsu, Yuki
Kimura, Yoshihiko
Katayose, Yoshihisa
Kamata, Shuichi
Terata, Kaori
Takahashi, Eriko
Ibonai, Ayano
Yamaguchi, Ayuko
Konno, Hikari
Yatsuyanagi, Misako
Kudo, Chiaki
Takashima, Shinogu
Akagami, Yoichi
Nakamura, Ryuta
Sato, Yusuke
Motoyama, Satoru
Nomura, Kyoko
Minamiya, Yoshihiro
|
| 内容記述 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Background: Human epidermal growth factor receptor 2-in situ hybridization (HER2-ISH) is widely approved for diagnostic, prognostic biomarker testing of formalin- fixed paraffin-embedded tissue blocks. However, cytologic ISH analysis has a potential advantage in tumor samples such as pleural effusion and ascites that are difficult to obtain the histological specimens. Our aim was to evaluate the clinical reliability of a novel rapid cytologic HER2 fluorescence ISH protocol (rapid-CytoFISH). Materials and Methods: Using a new device, we applied a high-voltage/frequency, noncontact alternating current electric field to tissue imprints and needle rinses, which mixed the probe within microdroplets as the voltage was switched on and off (AC mixing). Cytologic samples (n = 143) were collected from patients with immunohistochemically identified HER2 breast cancers. The specimens were then tested using standard dual-color ISH using formalin-fixed paraffin-embedded tissue (FFPE-tissue DISH) for HER2-targeted therapies, CytoFISH, and rapid-CytoFISH (completed within 4 h). Results: All 143 collected cytologic specimens (50 imprinted cytology specimens from resected tumors and 93 liquid-based cytology specimens from needle rinses) were suitable for FISH analysis. The HER2/chromosome enumeration probe (CEP) 17 ratios did not significantly differ between FFPE-tissue DISH and either CytoFISH protocol. Based on HER2 scoring criteria, we found 95.1% agreement between FFPEtissue DISH and CytoFISH (Cohen's kappa coefficient = 0.771 and 95% confidence interval (CI): 0.614–0.927). Conclusion: CytoFISH could potentially serve as a clinical tool for prompt determination of HER2 status in breast cancer cytology. Rapid-CytoFISH with AC mixing will enable cancer diagnoses and HER2 status to be determined on the same day a patient comes to a clinic or hospital. |
|
言語 |
en |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 書誌情報 |
en : Cancer Medicine
巻 10,
p. 586-594,
発行日 2021
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| 収録物識別子 |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2045-7634 |
| 出版者 |
|
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出版者 |
John Wiley & Sons |
|
言語 |
en |
| 関連情報 |
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関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1002/cam4.3626 |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
c2020 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
iA_2021_26 (731.4 kB)
