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Studies on Geranylgeranylacetone selectively binds to the HSP70 of Helicobacter pylori and alters its coccoid morphology
http://hdl.handle.net/10295/3107
http://hdl.handle.net/10295/31075581b1e0-ba86-4497-a54a-f3c4a7e4344e
| 名前 / ファイル | ライセンス | アクション |
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内容要旨及び審査結果要旨 (326.9 kB)
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本文 (1.9 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
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| 公開日 | 2016-06-22 | |||||||
| タイトル | ||||||||
| タイトル | Studies on Geranylgeranylacetone selectively binds to the HSP70 of Helicobacter pylori and alters its coccoid morphology | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 別タイトル | ||||||||
| その他のタイトル | Geranylgeranylacetoneのヘリコバクター ピロリ菌HSP70への選択的結合及び形態変化促進機構に関する研究 | |||||||
| 作成者 |
GRAVE, EWA
× GRAVE, EWA
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| 内容記述（抄録） | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Geranylgeranylacetone (GGA) is used to treat patients suffering from peptic ulcers and gastritis. We examined the effect of GGA on Helicobacter pylori, which is a causative factor of gastrointestinal diseases. Previously, we have reported that GGA binds specifically to the molecular chaperone HSP70. In this paper, we report that GGA bounds to H. pylori HSP70 (product of the DnaK gene) with 26-times higher affinity than to human HSP70, and induced large conformational changes as observed from surface plasmon resonance and circular dichroism. Binding of GGA suppressed the activity of the H. pylori chaperone. GGA also altered several characteristics of H. pylori cells. GGA-treated cells elicited enhanced interleukin-8 production by gastric cancer cell lines and potentiated susceptibility to complement as compared to untreated cells. GGA also caused morphological alterations in H. pylori as reflected in fewer coccoid-like cells, suggesting that GGA converts H. pylori to an actively dividing, spiral state (vegetative form) from a non-growing, coccoid state. This morphological conversion by GGA resulted in accelerated growth of H. pylori. These results suggest a model in which GGA sensitizes H. pylori to antibiotic treatment by converting the cells to an actively growing state. | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| DOI | ||||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | info:doi/10.1038/srep13738 | |||||||
| 書誌情報 | 発行日 2016-06-01 | |||||||
| 出版者 | ||||||||
| 出版者 | 秋田大学 | |||||||
| 学位名 | ||||||||
| 学位名 | 博士(理学) | |||||||
| 学位授与機関 | ||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||
| 学位授与機関識別子 | 11401 | |||||||
| 学位授与機関名 | 秋田大学 | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2016-03-22 | |||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲第1170号 | |||||||
