| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2014-06-27 |
| タイトル |
|
|
タイトル |
Functional Roles of TGF-β1 in intestinal epithelial cells through Smad-dependent and Non-Smad pathways |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 別タイトル |
|
|
その他のタイトル |
小腸粘膜上皮細胞におけるSmad 依存性経路，非依存性経路を介したTGF-β1 の役割 |
| 作成者 |
山田, 由美
Yamada, Yumi
|
| 内容記述（抄録） |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Background & Aims: Transforming growth factor-β_1 (TGF-β_1) is one of the growth factors expressed in the gut, and has been shown to play an important role in intestinal mucosal healing. We investigated the effects of TGF-β_1 on the cellular functions of intestinal epithelial cells, and also evaluated its signaling pathways in these cells. Methods: We used the rat IEC-6 intestinal epithelial cell line for these studies. The expression of TGF-β_1/Smad signaling molecules was examined. We evaluated the effect of TGF-β_1 on the proliferation and differentiation by the BrdU incorporation assay and real-time PCR. We manipulated the expression levels of Smad2 and Smad3 using an adenovirus system and small interfering RNA to examine the signaling pathways. The expression of Smad2 and Smad3 along the crypt-villus axis was also examined in the murine intestine. Results: IEC-6 cells produced TGF-β_1 and expressed functional TGF-β/Smad signaling molecules. The addition of TGF-β_1 in the culture medium suppressed the proliferation and increased the expression of a differentiation marker of enterocytes, in a dose-dependent manner. The adenovirus-mediated and small interfering RNA-mediated studies clearly showed that the growth inhibitory effect and the promotion of differentiation were exerted through a Smad3-dependent and a Smad2-dependent pathway, respectively. IEC-6 cells exhibited upregulated expression of an inhibitory Smad (Smad7) as a form of negative feedback via a non-Smad pathway. Smad2 was predominantly expressed in villi, and Smad3 in crypts. Conclusions: TGF-β_1 regulates the cellular functions of intestinal epithelial cells through both Smad-dependent and non-Smad pathways. |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
info:doi/10.1007/s10620-012-2515-7 |
| 書誌情報 |
発行日 2014-06-01
|
| 備考 |
|
|
|
秋田大学審査学位論文（Digestive Diseases and Sciences,58(5),p1207-p1217掲載論文の著者最終稿） |
| 学位名 |
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|
学位名 |
博士（医学） |
| 学位授与機関 |
|
|
|
学位授与機関識別子Scheme |
kakenhi |
|
|
学位授与機関識別子 |
11401 |
|
|
学位授与機関名 |
秋田大学 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2014-03-22 |
| 学位授与番号 |
|
|
学位授与番号 |
甲第1078号 |
本文 (5.1 MB)
