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TEMPORAL EXPRESSION OF FUNCTIONAL LOW DENSITY LIPOPROTEIN RECEPTORS AND CATALYTIC ENZYMES IN STEROID HORMONE SYNTHESIS OF MOUSE PREIMPLANTATION EMBRYOS
http://hdl.handle.net/10295/1989
http://hdl.handle.net/10295/19892d8283d8-e934-41fc-8f2c-1299a40ac0db
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
akitai39(1).pdf (2.6 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2012-10-21 | |||||
| タイトル | ||||||
| タイトル | TEMPORAL EXPRESSION OF FUNCTIONAL LOW DENSITY LIPOPROTEIN RECEPTORS AND CATALYTIC ENZYMES IN STEROID HORMONE SYNTHESIS OF MOUSE PREIMPLANTATION EMBRYOS | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 主題 | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Development | |||||
| 主題 | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | LDL | |||||
| 主題 | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | LDL receptor | |||||
| 主題 | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Preimplantation embryo | |||||
| 主題 | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Steroidogenesis | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 作成者 |
Sato, Naoki
× Sato, Naoki× Terada, Yukihiro |
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| 内容記述 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Mammalian preimplantation embryos, which lack a storage organ for cholesterol which plays an essential role in the composition of cell membranes may exhaust the cholesterol without uptake from lipoprotein during development. LDL is the major lipoprotein carrier in mammals. The aim of this study was to investigate the possibility of de novo steroidsynthesis in mouse preimplantation embryos. Using collected murine oocytes and embryos, the temporal expressions of either LDL receptor (LDLR) or enzymes to synthesize steroid hormones were analyzed at the mRNA level using RT-PCR techniques. The temporal expressions of LDLR and P450scc protein were analyzed by immunostaining. To monitor the uptake of LDL in embryos, an LDL labeled with a probe was used. LDLR mRNA was detected at the oocyte, 8-cell, morula, blastocyst, and hatched blastocyst stages. P450scc mRNA was detected at the oocyte, 1-cell, 2-cell, 4-cell, blastocyst and hatched blastocyst stages. β-HSD VI mRNA was detected at the oocyte, blastocyst and hatched blastocyst stages. P450arom mRNA was detected only at oocyte. The expression of LDLR protein was detected in trophectoderm cells of blastocysts, whereas P450scc was detected in cytoplasm of oocytes and all embryos. Labeled LDL was only taken in by blastocysts, but not oocytes or 4-cell stage embryos. These results suggest that murine blastocysts could utilize LDL as a source of cholesterol for de novo progesterone synthesis. | |||||
| 言語 | en | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 書誌情報 |
ja : 秋田医学 巻 39, 号 1, p. 1-12, 発行日 2012-09-28 |
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| 収録物識別子 | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 03866106 | |||||
| 収録物識別子 | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00009294 | |||||
| 出版者 | ||||||
| 出版者 | 秋田医学会 | |||||
| 言語 | ja | |||||
