| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2011-10-18 |
| タイトル |
|
|
タイトル |
INCREASING LEVELS OF AMPHIREGULIN IN FOLLICULAR FLUIDS ARE ASSOCIATED WITH HUMAN OOCYTE MATURATION |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
amphiregulin |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
EGF-like growth factors |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
follicular fluid |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
oocyte maturation |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 作成者 |
Sasaki, Mitsue
Kawamura, Kazuhiro
Yinghui, Ye
Kumazawa, Yukiyo
Kumagai, Jin
Tanaka, Toshinobu
|
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Luteinizing hormone stimulates ovarian somatic cells to induce hormone release for oocyte maturation. Accumulating evidences in rodents indicate importance of epidermal growth factor (EGF)-like growth factors [amphiregulin (AREG), epiregulin (EREG) and betacellulin (BTC)] from the somatic cells in oocyte maturation. Here, we investigated an involvement of ovarian EGF-like growth factors in human oocyte maturation. Using isolated human ovarian cells, we found the expression of AREG, EREG and BTC mRNAs in granulosa and cumulus cells by RT-PCR, and AREG protein in cumulus cells, suggesting their production in ovarian somatic cells. In human follicular fluids aspirated from individual follicles undergoing in vitro fertilization-embryo transfer and granulosa cells obtained by centrifugation of the fluid, the association between AREG, EREG, and BTC levels and oocyte maturation was examined by using enzyme-linked immunosorbent assay or quantitative real-time RT-PCR................................. ...............................AREG protein level was significantly higher in the follicular fluids that yielded mature oocytes. However, EREG mRNA level in granulosa cells was insignificant between mature and immature oocytes, and BTC protein was absent from all follicular fluids examined. Our findings suggest an important role for AREG, but not EREG and BTC, in optimal maturation of human oocytes. |
|
言語 |
en |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 書誌情報 |
ja : 秋田医学
巻 37,
号 1,
p. 43-50,
発行日 2010-01-01
|
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
03866106 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00009294 |
| 出版者 |
|
|
出版者 |
秋田医学会 |
|
言語 |
ja |
akitai37(43).pdf (1.0 MB)
