WEKO3
アイテム
半定量的RT-PCR法を用いたマウス網膜における抗原提示機能の可能性と解析
http://hdl.handle.net/10295/1548
http://hdl.handle.net/10295/1548d121601b-cdcd-4d52-93f3-ba7b11d6b5cd
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
akigaku31-2f.pdf (1.3 MB)
|
|
| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2009-01-19 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | 半定量的RT-PCR法を用いたマウス網膜における抗原提示機能の可能性と解析 | |||||||||||||||||
| 言語 | ja | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | The use of semi-quantitative Reverse Transcription-Polymerase Chain Reaction (RT-PCR) to analyze antigen-presenting functions in mouse retina | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | jpn | |||||||||||||||||
| 主題 | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Semi-quantitative RT-PCR | |||||||||||||||||
| 主題 | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Toll-like receptor | |||||||||||||||||
| 主題 | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Antigen-presenting cells | |||||||||||||||||
| 主題 | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Immune privilege site | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||
| アクセス権 | ||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||||
| 作成者 |
原, 宏二
× 原, 宏二
× 亀谷, 修平
× 山木, 邦比古
|
|||||||||||||||||
| 内容記述 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | To identify the possibility of antigen presentation in the retina, we studied the production of various cytokines and the expression of cell surface molecules in the retina by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Lipopolysaccharide (LPS) or inactivated Bordetella pertussis (BP) was injected into the vitreous cavity of C57BL/6J mice. The retinas were collected 3,6 and 24 hours after the injection. Expression of mRNA for MHC class II, CDllc, CDSO, CDS6, toll-like receptor (TLR) 4, TLR9, IL-12p35, IL-2, IFN-y, IL-4, IL-5, IL-6, IL-IO, IL-lj3, TNF-a and TGF-j3 was analyzed by semi-quantitative RT-PCR. Expression of mRNA for MHC class II, CDllc, CD80, CDS6, TLR4 and TLR9 was found in the untreated retina. The amount of expression of MHC class II, CDllc, CDSO and CD86 increased after injection of either LPS or BP. Expression of TLR4 mRNA increased only following injection with LPS, whereas TLR9 increased following injection with BP or LPS, not clear. The expression of cytokines, IL-12p35, IL-2, IFN-y, IL-4, IL-5, IL-6, IL-IO, IL-lj3, TNF-a and TGF-j3 increased after injection with either LPS or BP. A time course of mRNA expression indicated a peak at 3 hours after injection, which then gradually subsided. These results suggest that proinflammatory cytokines related to antigen presentation were upregulated by stimulation of TLR4 and TLR9. | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 出版タイプ | ||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
| 書誌情報 |
秋田医学 巻 31, 号 2, p. 141-152, 発行日 2004-09-01 |
|||||||||||||||||
| 収録物識別子 | ||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 03866106 | |||||||||||||||||
| 収録物識別子 | ||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||
| 収録物識別子 | AN00009294 | |||||||||||||||||
| 出版者 | ||||||||||||||||||
| 出版者 | 秋田医学会 | |||||||||||||||||
