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Live visualisation of electrolytes during mouse embryonic development using electrolyte indicators
http://hdl.handle.net/10295/00006313
http://hdl.handle.net/10295/00006313d53a3b3b-bb85-4041-8184-1068114c21d2
| 名前 / ファイル | ライセンス | アクション |
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iA_2022_120.pdf (1.8 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||||||||||
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| 公開日 | 2023-02-25 | |||||||||||||||||||||||||||||
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| タイトル | Live visualisation of electrolytes during mouse embryonic development using electrolyte indicators | |||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||||||||||||||||
| 作成者 |
Fujishima, Akiko
× Fujishima, Akiko
× Takahashi, Kazumasa
× Goto, Mayumi
× Hirakawa, Takeo
× Iwasawa, Takuya
× Togashi, Kazue
× Maeda, Eri
× Shirasawa, Hiromitsu
× Miura, Hiroshi
× Sato, Wataru
× Kumazawa, Yukiyo
× Terada, Yukihiro
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| 内容記述 | ||||||||||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||||||||||
| 内容記述 | Studies have shown that some electrolytes, including Na+ and K+, play important roles in embryonic development. However, these studies evaluated these electrolytes by using inhibitors or knockout mice, with no mention on the changes in the intracellular electrolyte concentrations during embryogenesis. In this study, we used the electrolyte indicators CoroNa Green AM and ION Potassium Green-2 AM to directly visualise intracellular concentrations of Na+ and K+, respectively, at each embryonic developmental stage in mouse embryos. We directly observed intracellular electrolyte concentrations at the morula, blastocyst, and hatching stages. Our results revealed dynamic changes in intracellular electrolyte concentrations; we found that the intracellular Na+ concentration decreased, while K+ concentration increased during blastocoel formation. The degree of change in intensity in response to ouabain, an inhibitor of Na+/K+ ATPase, was considered to correspond to the degree of Na+/K+ ATPase activity at each developmental stage. Additionally, after the blastocyst stage, trophectoderm cells in direct contact with the blastocoel showed higher K+ concentrations than in direct contact with inner cell mass, indicating that Na+/K+ ATPase activity differs depending on the location in the trophectoderm. This is the first study to use CoroNa Green AM and ION Potassium Green-2 AM in mouse embryos and visualise electrolytes during embryonic development. The changes in electrolyte concentration observed in this study were consistent with the activity of Na+/K+ ATPase reported previously, and it was possible to image more detailed electrolyte behaviour in embryo cells. This method can be used to improve the understanding of cell physiology and is useful for future embryonic development studies. | |||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||||||||
| 書誌情報 |
en : PLOS ONE 巻 16, 号 1, 発行日 2021 |
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| 収録物識別子 | ||||||||||||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||||||||
| 収録物識別子 | 1932-6203 | |||||||||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||||||||
| 出版者 | Public Library of Science | |||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||||
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| 関連タイプ | isIdenticalTo | |||||||||||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1371/journal.pone.0246337 | |||||||||||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||||||||||
| 権利情報 | © 2021 Fujishima et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||||||||||||||||||||||||||
