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MCP-1 INHIBITS DNA SYNTHESIS IN RAT PANCREATIC STELLATE CELLS
http://hdl.handle.net/10295/1710
http://hdl.handle.net/10295/171001b1d7ca-bee4-4418-9680-8ad87bab7565
名前 / ファイル | ライセンス | アクション |
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akitai36(185).pdf (504.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2011-06-23 | |||||
タイトル | ||||||
タイトル | MCP-1 INHIBITS DNA SYNTHESIS IN RAT PANCREATIC STELLATE CELLS | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | MCP-1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | CCR2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pancreatic stellate cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA replication | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Autocrine | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Paracrine | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
KAMADA, Kentaro
× KAMADA, Kentaro× MASHIMA, Hirosato× GOTO, Takashi× OHNISHI, Hirohide |
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内容記述(抄録) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Activated pancreatic stellate cells (PSCs) synthesize various kinds of cytokines and chemokines including monocyte chemoattractant protein-1 (MCP-1) and play major roles in promoting inflammation and fibrogenesis in the pancreas. MCP-1 is a potent chemotactic factor for leukocytes and it has recently been shown that the target is not restricted. The aim of this study was to investigate whether MCP-1 exerts a biological effect on PSCs. Cultured rat PSCs secreted MCP1 independent of the concentration of transforming growth factor-β1 (TGF-β 1) in the culture media. Although PSCs lack the typical receptor system (C-C chemokine receptor 2 (CCR2)), MCP-1 inhibited DNA synthesis in PSCs without activation, suggesting the presence of CCR2-independent MCP-1 signaling pathway. Further, MCP-1 inhibited the proliferation of PSCs in which TGF-β 1/Smad pathway was blocked by the dominant-negative Smad2/3 over-expression. MCP-1 did not affect the phosphorylation state of mitogen-activated protein kinase (MAPK), Akt, nor epidermal growth factor receptor (EGFR). Taken together, MCP-1 inhibited DNA synthesis of cultured rat PSCs in an autocrine or paracrine manner without activation and this effect was exerted through CCR2-independent and TGF-β1/Smad-independent pathway. These data provide new insights to better understand MCP-1 participation in pancreatic inflammation and also to develop a new strategy for its treatment. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
書誌情報 |
秋田医学 巻 36, 号 3-4, p. 185-194, 発行日 2010-03-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03866106 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00009294 | |||||
出版者 | ||||||
出版者 | 秋田医学会 |