{"created":"2023-07-25T10:22:20.266369+00:00","id":1673,"links":{},"metadata":{"_buckets":{"deposit":"22750913-3acc-4014-b582-2c405b76166a"},"_deposit":{"created_by":15,"id":"1673","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"1673"},"status":"published"},"_oai":{"id":"oai:air.repo.nii.ac.jp:00001673","sets":["611:862:863:881"]},"author_link":["6133","6134","6135","6136"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3-4","bibliographicPageEnd":"194","bibliographicPageStart":"185","bibliographicVolumeNumber":"36","bibliographic_titles":[{"bibliographic_title":"秋田医学","bibliographic_titleLang":"ja"}]}]},"item_10001_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Activated pancreatic stellate cells (PSCs) synthesize various kinds of cytokines and chemokines including monocyte chemoattractant protein-1 (MCP-1) and play major roles in promoting inflammation and fibrogenesis in the pancreas. MCP-1 is a potent chemotactic factor for leukocytes and it has recently been shown that the target is not restricted. The aim of this study was to investigate whether MCP-1 exerts a biological effect on PSCs. Cultured rat PSCs secreted MCP1 independent of the concentration of transforming growth factor-β1 (TGF-β 1) in the culture media. Although PSCs lack the typical receptor system (C-C chemokine receptor 2 (CCR2)), MCP-1 inhibited DNA synthesis in PSCs without activation, suggesting the presence of CCR2-independent MCP-1 signaling pathway. Further, MCP-1 inhibited the proliferation of PSCs in which TGF-β 1/Smad pathway was blocked by the dominant-negative Smad2/3 over-expression. MCP-1 did not affect the phosphorylation state of mitogen-activated protein kinase (MAPK), Akt, nor epidermal growth factor receptor (EGFR). Taken together, MCP-1 inhibited DNA synthesis of cultured rat PSCs in an autocrine or paracrine manner without activation and this effect was exerted through CCR2-independent and TGF-β1/Smad-independent pathway. These data provide new insights to better understand MCP-1 participation in pancreatic inflammation and also to develop a new strategy for its treatment.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"秋田医学会","subitem_publisher_language":"ja"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1724099666130":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"03866106","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AN00009294","subitem_source_identifier_type":"NCID"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"KAMADA, Kentaro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6133","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"MASHIMA, Hirosato","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6134","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"GOTO, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6135","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"OHNISHI, Hirohide","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6136","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-16"}],"displaytype":"detail","filename":"akitai36(185).pdf","filesize":[{"value":"504.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"akitai36(185).pdf","url":"https://air.repo.nii.ac.jp/record/1673/files/akitai36(185).pdf"},"version_id":"105fd750-6438-4559-b243-aaf59e26625b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"MCP-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"CCR2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Pancreatic stellate cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA replication","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Autocrine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Paracrine","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"MCP-1 INHIBITS DNA SYNTHESIS IN RAT PANCREATIC STELLATE CELLS","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"MCP-1 INHIBITS DNA SYNTHESIS IN RAT PANCREATIC STELLATE CELLS","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"15","path":["881"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2011-06-23"},"publish_date":"2011-06-23","publish_status":"0","recid":"1673","relation_version_is_last":true,"title":["MCP-1 INHIBITS DNA SYNTHESIS IN RAT PANCREATIC STELLATE CELLS"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-08-25T08:47:00.833527+00:00"}