{"created":"2023-07-25T10:21:33.907392+00:00","id":618,"links":{},"metadata":{"_buckets":{"deposit":"487185ec-dba6-49c2-ae90-b9349257f500"},"_deposit":{"created_by":3,"id":"618","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"618"},"status":"published"},"_oai":{"id":"oai:air.repo.nii.ac.jp:00000618","sets":["611:612:613:619"]},"author_link":["2150","2146","2145","2148","2149","2147"],"item_10002_alternative_title_34":{"attribute_name":"別タイトル","attribute_value_mlt":[{"subitem_alternative_title":"Basic Concept of Radical Oxygen Species Studies with Reference to Studies on Male Infertility"}]},"item_10002_biblio_info_36":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-10-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"30","bibliographicPageStart":"23","bibliographicVolumeNumber":"13","bibliographic_titles":[{"bibliographic_title":"秋田大学医学部 保健学科紀要"}]}]},"item_10002_description_29":{"attribute_name":"内容記述（抄録）","attribute_value_mlt":[{"subitem_description":"活性酸素によりもたらされる酸化ストレスによる細胞障害は,近年の医学研究の大きな関心事である.本稿では,活性酸素に対する基礎的な知識を解説するとともに,活性酸素に関するいくつかの実験主義について,著者が経験した男性不妊の実験モデルを例に解説した.培養系で活性酸素の細胞への影響を調べる場合,活性酸素産生酵素がしばしば使用される.臓器や生体の活性酸素のレベルを知りたい場合,活性酸素により障害を受けた物質の量(過酸化脂質や8-ヒドロキシデオグアノシンなど)を測定し,間接的に活性酸素のレベルを評価する.ある生物現象に酸化ストレスが関与するかを検討したい場合の最も基本的なアプローチは,活性酸素消去酵素や活性酸素の産生の抑制剤によりその現象が影響を受けるかどうか検討することである.通常活性酸素は細胞にとって有害な分子であるが,様々な生物作用において生理的な作用も示すので,注意が必要である.","subitem_description_type":"Other"},{"subitem_description":"Recently a number of medical studies have focused on cell damage induced by radical oxygen species (ROS) attack, so-called oxidative stress. This review describes basic knowledge on ROS and the concept of some experimental methods relating to ROS, making reference to our studies on male infertility. The ROS generating enzymes are sometimes used to investigate the effect of ROS on various cells in a culture system. The levels of ROS in tissues or bodies can be measured indirectly by detection of products of ROS-mediated reactions, such as fatty acid peroxide or 8-hydrox-2'-deoxyguanosine. Whether ROS is involved in a certain biological process can be investigated by experiment using inhibitors of ROS generations or ROS scavengers. Although the effects of ROS are usually deleterious to cells, we should be cautious as they are sometimes useful for various biological processes.","subitem_description_type":"Other"}]},"item_10002_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"秋田大学医学部"}]},"item_10002_source_id_27":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13478664","subitem_source_identifier_type":"ISSN"}]},"item_10002_source_id_35":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA11834061","subitem_source_identifier_type":"NCID"}]},"item_10002_text_25":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"秋田大学医学部保健学科看護学専攻"},{"subitem_text_value":"秋田大学医学部保健学科看護学専攻"},{"subitem_text_value":"秋田大学医学部保健学科看護学専攻"}]},"item_10002_version_type_37":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"兒玉, 英也"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"渡邉, 竹美"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"糠塚, 亜紀子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KODAMA, Hideya","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"WATANABE, Takemi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"NUKAZUKA, Akiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-16"}],"displaytype":"detail","filename":"KJ00004185091.pdf","filesize":[{"value":"779.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00004185091.pdf","url":"https://air.repo.nii.ac.jp/record/618/files/KJ00004185091.pdf"},"version_id":"ef791724-5a1a-4d53-a6a8-b8db077ad3e8"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"活性酸素","subitem_subject_scheme":"Other"},{"subitem_subject":"酸化的ストレス","subitem_subject_scheme":"Other"},{"subitem_subject":"男性不妊","subitem_subject_scheme":"Other"},{"subitem_subject":"活性酸素産生反応","subitem_subject_scheme":"Other"},{"subitem_subject":"活性酸素消去酵素","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"活性酸素に関する実験系の基礎的概念について : 男性不妊の実験モデルを参考に","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"活性酸素に関する実験系の基礎的概念について : 男性不妊の実験モデルを参考に"}]},"item_type_id":"10002","owner":"3","path":["619"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-12-14"},"publish_date":"2007-12-14","publish_status":"0","recid":"618","relation_version_is_last":true,"title":["活性酸素に関する実験系の基礎的概念について : 男性不妊の実験モデルを参考に"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-25T12:04:52.397644+00:00"}