{"created":"2023-07-25T10:25:35.287422+00:00","id":6000,"links":{},"metadata":{"_buckets":{"deposit":"56e436f7-3d7d-49fa-adbf-bbf946574ad0"},"_deposit":{"created_by":3,"id":"6000","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"6000"},"status":"published"},"_oai":{"id":"oai:air.repo.nii.ac.jp:00006000","sets":["611:939:940"]},"author_link":["18074","18075","18077","18073","18072","18070","18071","18076"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"PLOS ONE"}]}]},"item_10001_description_5":{"attribute_name":"内容記述（抄録）","attribute_value_mlt":[{"subitem_description":"Recent studies reported morphokinetic indices for optimal selection of embryos in assisted reproductive technology (ART). The morphokinetics in blastocyst stage include the collapse and re-expansion rates after thawing. However, evaluation methods using these morphokinetics have not been established, mainly because the underlying molecular mechanisms remain unclarified. In this study, we focused on the relationship between these morphokinetic observation of the blastocyst behaviour and the number of cells constituting the blastocyst. We evaluated 38 surplus human frozen-thawed blastocysts using time-lapse cinematography and recorded their expansion, contraction, and hatching. A total of 28 blastocysts expanded in culture (cross-sectional area >= 5,000 Pi mu m(2)). In comparison to the ones that did not, the expanded group presented significantly more number of inner cell mass (ICM) and trophectoderm (TE) cells, which eventually develop into the fetus and placenta, respectively (ICM: Expanded 10.2 +/- 6.3 vs. Non-Expanded 6.0 +/- 12.3, p<0.05; TE: Expanded 165.7 +/- 74.8 vs. Non-Expanded 57.0 +/- 29.4, p<0.05). Moreover, a positive correlation was found between the expansion rate (up to 4 h) and the number of TE cells (r=0.558, p=0.0021). Additionally, blastocysts that hatched had a significantly higher number of TE cells than those that did not (hatching 225.2 +/- 61.2 vs. no hatching 121.1 +/- 48.6, p<0.0001). The number of TE cells per unit of cross-sectional area correlated negatively with the contraction time (r=-0.601, p=0.0007). No correlation between the number of ICM cells and these morphokinetics was detected. In conclusion, our study demonstrates that different morphokinetics of frozen-thawed blastocysts reflect the number of TE cells. The differentiation of blastocysts containing sufficient TE cells would be beneficial for implantation and prognosis of a subsequent pregnancy. Thus, evaluation of these morphokinetics can be an effective method to screen good embryos for ART.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0210992","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_25":{"attribute_name":"関連リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://dx.doi.org/10.1371/journal.pone.0210992"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1371/journal.pone.0210992","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"著作権等","attribute_value_mlt":[{"subitem_rights":"© 2019 Iwasawa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1932-6203","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Iwasawa, Takuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Kazumasa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Goto, Mayumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Anzai, Mibuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shirasawa, Hiromitsu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sato, Wataru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kumazawa, Yukiyo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Terada, Yukihiro"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-25"}],"displaytype":"detail","filename":"iA_2022_119.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"iA_2022_119.pdf","url":"https://air.repo.nii.ac.jp/record/6000/files/iA_2022_119.pdf"},"version_id":"eedba433-806d-4688-bbbd-58c55708d48d"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Human frozen-thawed blastocyst morphokinetics observed using time-lapse cinematography reflects the number of trophectoderm cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Human frozen-thawed blastocyst morphokinetics observed using time-lapse cinematography reflects the number of trophectoderm cells"}]},"item_type_id":"10001","owner":"3","path":["940"],"pubdate":{"attribute_name":"公開日","attribute_value":"2023-02-25"},"publish_date":"2023-02-25","publish_status":"0","recid":"6000","relation_version_is_last":true,"title":["Human frozen-thawed blastocyst morphokinetics observed using time-lapse cinematography reflects the number of trophectoderm cells"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-25T10:38:41.754004+00:00"}