{"created":"2023-07-25T10:25:34.415821+00:00","id":5972,"links":{},"metadata":{"_buckets":{"deposit":"27cd986a-0848-4822-8040-99082b3920d4"},"_deposit":{"created_by":3,"id":"5972","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5972"},"status":"published"},"_oai":{"id":"oai:air.repo.nii.ac.jp:00005972","sets":["611:939:940"]},"author_link":["17866","17869","17873","17876","17861","17872","17863","17874","17864","17871","17877","17862","17867","17868","17870","17875","17859","17865","17860","17878"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"SCIENTIFIC REPORTS"}]}]},"item_10001_description_5":{"attribute_name":"内容記述(抄録)","attribute_value_mlt":[{"subitem_description":"Recent genomic studies on cancer tissues obtained during rapid autopsy have provided insights into the clonal evolution and heterogeneity of cancer. However, post-mortem blood has not been subjected to genetic analyses in relation to cancer. We first confirmed that substantial quantities of cell-free DNA were present in the post-mortem plasma of 12 autopsy cases. Then, we focused on a pilot case of prostate cancer with multiple metastases for genetic analyses. Whole-exome sequencing of post-mortem plasma-derived cell-free DNA and eight frozen metastatic cancer tissues collected during rapid autopsy was performed, and compared their mutational statuses. The post-mortem plasma cell-free DNA was successfully sequenced and 344 mutations were identified. Of these, 160 were detected in at least one of the metastases. Further, 99% of the mutations shared by all metastases were present in the plasma. Sanger sequencing of 30 additional formalin-fixed metastases enabled us to map the clones harboring mutations initially detected only in the plasma. In conclusion, post-mortem blood, which is usually disposed of during conventional autopsies, can provide valuable data if sequenced in detail, especially regarding cancer heterogeneity. Furthermore, post-mortem plasma cell-free DNA sequencing (liquid autopsy) can be a novel platform for cancer research and a tool for genomic pathology.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature Research"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/s41598-020-59193-y","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_25":{"attribute_name":"関連リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://dx.doi.org/10.1038/s41598-020-59193-y"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1038/s41598-020-59193-y","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"著作権等","attribute_value_mlt":[{"subitem_rights":"Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2020"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2045-2322","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takai, Erina"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Maeda, Daichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Li, Zhuo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kudo-Asabe, Yukitsugu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Totoki, Yasushi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Hiromi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Akiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Rumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kirikawa, Misato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ito, Yukinobu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshida, Makoto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Inoue, Takamitsu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Habuchi, Tomonori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ikoma, Shohei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Katoh, Hiroto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kato, Mamoru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shibata, Tatsuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishikawa, Shumpei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yachida, Shinichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Goto, Akiteru"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-25"}],"displaytype":"detail","filename":"iA_2022_110.pdf","filesize":[{"value":"3.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"iA_2022_110.pdf","url":"https://air.repo.nii.ac.jp/record/5972/files/iA_2022_110.pdf"},"version_id":"02d0b42a-5609-4fb4-8111-f8e5fb97bbc4"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Post-mortem Plasma Cell-Free DNA Sequencing: Proof-of-Concept Study for the Liquid Autopsy","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Post-mortem Plasma Cell-Free DNA Sequencing: Proof-of-Concept Study for the Liquid Autopsy"}]},"item_type_id":"10001","owner":"3","path":["940"],"pubdate":{"attribute_name":"公開日","attribute_value":"2023-02-25"},"publish_date":"2023-02-25","publish_status":"0","recid":"5972","relation_version_is_last":true,"title":["Post-mortem Plasma Cell-Free DNA Sequencing: Proof-of-Concept Study for the Liquid Autopsy"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-25T10:38:59.283140+00:00"}