{"created":"2023-07-25T10:25:34.064973+00:00","id":5964,"links":{},"metadata":{"_buckets":{"deposit":"475e1b89-b822-47b5-82f3-905366f82255"},"_deposit":{"created_by":3,"id":"5964","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5964"},"status":"published"},"_oai":{"id":"oai:air.repo.nii.ac.jp:00005964","sets":["611:939:940"]},"author_link":["17804","17801","17803","17795","17797","17800","17794","17796","17799","17802","17798"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"SCIENTIFIC REPORTS"}]}]},"item_10001_description_5":{"attribute_name":"内容記述（抄録）","attribute_value_mlt":[{"subitem_description":"Primary immune thrombocytopenia (ITP) is an acquired form of thrombocytopenia caused by IgG anti-platelet autoantibodies and represents an organ-specific autoimmune disorder. Although the glycoprotein (GP) IIb/IIIa and GPIb/IX have been shown to be targets for autoantibodies, the antigen specificity of autoantibodies is not fully elucidated. To identify the characteristics of IgG B-cell receptor (BCR) repertoires in ITP, we took advantage of adaptor-ligation PCR and high-throughput DNA sequencing methods for analyzing the clone-based repertoires of IgG-expressing peripheral blood B cells. A total of 2,009,943 in-frame and 315,469 unique reads for IGH (immunoglobulin heavy) were obtained from twenty blood samples. Comparison of the IGHV repertoires between patients and controls revealed an increased usage of IGHV4-28 in ITP patients. One hundred eighty-six distinct IGHV4-28-carrying sequences were identified in ITP patients and the majority of these clones used an IGHJ4 segment. The IGHV4-28/IGHJ4-carrying B-cell clones were found in all ITP patients. Oligoclonal expansions of IGHV4-28/IGHJ4-carrying B cells were accompanied by multiple related clones with single amino substitution in the CDR3 region suggesting somatic hypermutation. Taken together, the expansion of IGHV4-28/IGHJ4-carrying IgG-expressing B cells in ITP may be the result of certain antigenic pressure and may provide a clue for the immune pathophysiology of ITP.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature Research"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/s41598-019-45264-2","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_25":{"attribute_name":"関連リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://dx.doi.org/10.1038/s41598-019-45264-2"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1038/s41598-019-45264-2","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"著作権等","attribute_value_mlt":[{"subitem_rights":"Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2019"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2045-2322","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hirokawa, Makoto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujishima, Naohito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Togashi, Masaru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saga, Akiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Omokawa, Ayumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saga, Tomoo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Moritoki, Yuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ueki, Shigeharu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Naoto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitaura, Kazutaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Ryuji"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-25"}],"displaytype":"detail","filename":"iA_2022_102.pdf","filesize":[{"value":"2.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"iA_2022_102.pdf","url":"https://air.repo.nii.ac.jp/record/5964/files/iA_2022_102.pdf"},"version_id":"99feb98a-8f18-4a68-b21a-e643525ba58e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"High-throughput sequencing of IgG B-cell receptors reveals frequent usage of the rearranged IGHV4-28/IGHJ4 gene in primary immune thrombocytopenia","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"High-throughput sequencing of IgG B-cell receptors reveals frequent usage of the rearranged IGHV4-28/IGHJ4 gene in primary immune thrombocytopenia"}]},"item_type_id":"10001","owner":"3","path":["940"],"pubdate":{"attribute_name":"公開日","attribute_value":"2023-02-25"},"publish_date":"2023-02-25","publish_status":"0","recid":"5964","relation_version_is_last":true,"title":["High-throughput sequencing of IgG B-cell receptors reveals frequent usage of the rearranged IGHV4-28/IGHJ4 gene in primary immune thrombocytopenia"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-25T10:39:06.469462+00:00"}