{"created":"2023-07-25T10:25:33.890066+00:00","id":5960,"links":{},"metadata":{"_buckets":{"deposit":"f4cd8b24-6c19-4fce-b3a0-9c5d9d6f2f11"},"_deposit":{"created_by":15,"id":"5960","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"5960"},"status":"published"},"_oai":{"id":"oai:air.repo.nii.ac.jp:00005960","sets":["611:939:940"]},"author_link":[],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"7","bibliographic_titles":[{"bibliographic_title":"SCIENTIFIC REPORTS","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Alu elements are primate-specific short interspersed elements (SINEs), over 1 million copies of which are present in the human genome; thus, Alu elements are useful targets for detecting human cells. However, previous Alu-based techniques for detecting human genomic DNA do not reach the theoretical limits of sensitivity and specificity. In this study, we developed a highly sensitive and specific Alu-based real-time PCR method for discriminating human cells from rodent cells, using a primer and probe set carefully designed to avoid possible cross-reactions with rodent genomes. From 100 ng of mixed human and rodent genomes, 1 fg of human genome, equivalent to 1 human cell in 100 million rodent cells, was detectable. Furthermore, in vivo mouse subrenal capsule xenotransplantation assays revealed that 10 human cells per mouse organ were detectable. In addition, after intravenous injection of human mesenchymal stem cells into NOD/SCID mice via tail vein, the biodistribution of human cells was trackable in the mouse lungs and kidneys for at least 1 week. Our findings indicate that our primer and probe set is applicable for the quantitative detection of tiny amounts of human cells, such as xenotransplanted human cancer or stem cells, in rodents.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature Research","subitem_publisher_language":"en"}]},"item_10001_relation_25":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/s41598-017-13402-3","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2017"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1724099666130":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"2045-2322","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Funakoshi, Kodai","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Bagheri, Mozhdeh","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Zhou, Ming","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Suzuki, Ryoji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Abe, Hiroshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Akashi, Hideo","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-25"}],"displaytype":"detail","filename":"iA_2022_98.pdf","filesize":[{"value":"1.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"iA_2022_98.pdf","url":"https://air.repo.nii.ac.jp/record/5960/files/iA_2022_98.pdf"},"version_id":"405b39d5-d8b6-46b4-a265-9b90b757f69e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Highly sensitive and specific Alu-based quantification of human cells among rodent cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Highly sensitive and specific Alu-based quantification of human cells among rodent cells","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"15","path":["940"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-02-25"},"publish_date":"2023-02-25","publish_status":"0","recid":"5960","relation_version_is_last":true,"title":["Highly sensitive and specific Alu-based quantification of human cells among rodent cells"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-08-22T23:15:22.121840+00:00"}