{"created":"2023-07-25T10:25:33.709869+00:00","id":5956,"links":{},"metadata":{"_buckets":{"deposit":"fa42fa76-048f-40fb-ac36-f8effa51f02b"},"_deposit":{"created_by":3,"id":"5956","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5956"},"status":"published"},"_oai":{"id":"oai:air.repo.nii.ac.jp:00005956","sets":["611:939:940"]},"author_link":["17735","17737","17732","17729","17736","17731","17733","17734","17730"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"SCIENTIFIC REPORTS"}]}]},"item_10001_description_5":{"attribute_name":"内容記述（抄録）","attribute_value_mlt":[{"subitem_description":"The molecular mechanisms involved in the terminal differentiation of erythroblasts have been elucidated by comparing enucleation and cell division. Although various similarities and differences between erythroblast enucleation and cytokinesis have been reported, the mechanisms that control enucleation remain unclear. We previously reported that dynein and microtubule-organizing centers mediated the polarization of nuclei in human erythroblasts. Moreover, the accumulation of F-actin was noted during the enucleation of erythroblasts. Therefore, during enucleation, upstream effectors in the signal transduction pathway regulating dynein or actin, such as cell division control protein 42 homolog (Cdc42), may be crucial. We herein investigated the effects of the Cdc42 inhibitor, CASIN, on cytokinesis and enucleation in colony-forming units-erythroid (CFU-Es) and mature erythroblasts (day 10). CASIN blocked the proliferation of CFU-Es and their enucleation in a dose-dependent manner. Dynein adopted an island-like distribution in the cytoplasm of non-treated CFU-Es, but was concentrated near the nucleus as a dot and co-localized with gamma -tubulin in CASIN-treated cells. CASIN blocked the accumulation of F-actin in CFU-Es and day 10 cells. These results demonstrated that Cdc42 plays an important role in cytokinesis, nuclear polarization and nuclear extrusion through a relationship with dynein and actin filament organization during the terminal differentiation of erythroblasts.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature Research"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/s41598-020-68799-1","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_25":{"attribute_name":"関連リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://dx.doi.org/10.1038/s41598-020-68799-1"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1038/s41598-020-68799-1","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"著作権等","attribute_value_mlt":[{"subitem_rights":"Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2020"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2045-2322","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ubukawa, Kumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Goto, Tatsufumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Asanuma, Ken"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sasaki, Yumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Guo, Yong-Mei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayashi, Isuzu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sawada, Kenichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wakui, Hideki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Naoto"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-25"}],"displaytype":"detail","filename":"iA_2022_94.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"iA_2022_94.pdf","url":"https://air.repo.nii.ac.jp/record/5956/files/iA_2022_94.pdf"},"version_id":"7ef4abcf-95ad-4347-aef9-ae9f9d7d06cb"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cdc42 regulates cell polarization and contractile actomyosin rings during terminal differentiation of human erythroblasts","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cdc42 regulates cell polarization and contractile actomyosin rings during terminal differentiation of human erythroblasts"}]},"item_type_id":"10001","owner":"3","path":["940"],"pubdate":{"attribute_name":"公開日","attribute_value":"2023-02-25"},"publish_date":"2023-02-25","publish_status":"0","recid":"5956","relation_version_is_last":true,"title":["Cdc42 regulates cell polarization and contractile actomyosin rings during terminal differentiation of human erythroblasts"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-25T10:39:12.378041+00:00"}