{"created":"2023-07-25T10:25:33.622948+00:00","id":5954,"links":{},"metadata":{"_buckets":{"deposit":"4a8d01d4-e9e8-402c-a4c6-cbbc4207ffbf"},"_deposit":{"created_by":15,"id":"5954","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"5954"},"status":"published"},"_oai":{"id":"oai:air.repo.nii.ac.jp:00005954","sets":["611:939:940"]},"author_link":[],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"SCIENTIFIC REPORTS","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Human epidermal growth factor receptor 2 (HER2)-targeted agents are an effective approach to treating HER2-positive breast cancer patients. However, the lack of survival benefit in HER2-negative patients as well as the toxic effects and high cost of the drugs highlight the need for accurate and prompt assessment of HER2 status. Our aim was to evaluate the clinical utility of a novel rapid dual in-situ hybridization (RISH) method developed to facilitate hybridization. The method takes advantage of the non-contact mixing effect of an alternating current (AC) electric field. One hundred sixty-three specimens were used from patients diagnosed with primary breast cancers identified immunohistochemically as HER2 0/1(+), (2+) or (3+). The specimens were all tested using conventional dual in-situ hybridization (DISH), DISH with an automated slide stainer, and RISH. With RISH the HER2 test was completed within 6 h, as compared to 20-22 h needed for the standard protocol. Although RISH produced results more promptly using smaller amounts of labeled antibody, the staining and accuracy of HER2 status evaluation with RISH was equal to or greater than with DISH. These results suggest RISH could be used as a clinical tool to promptly determine HER2 status.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature Research","subitem_publisher_language":"en"}]},"item_10001_relation_25":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/srep30034","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1724099666130":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"2045-2322","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Saito, Yoshitaro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Imai, Kazuhiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nakamura, Ryuta","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nanjo, Hiroshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Terata, Kaori","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Konno, Hayato","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Akagami, Yoichi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Minamiya, Yoshihiro","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-25"}],"displaytype":"detail","filename":"iA_2022_92.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"iA_2022_92.pdf","url":"https://air.repo.nii.ac.jp/record/5954/files/iA_2022_92.pdf"},"version_id":"c99c1da6-6759-48da-ba48-99002c1260e4"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Novel method for rapid in-situ hybridization of HER2 using non-contact alternating-current electric-field mixing","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Novel method for rapid in-situ hybridization of HER2 using non-contact alternating-current electric-field mixing","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"15","path":["940"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-02-25"},"publish_date":"2023-02-25","publish_status":"0","recid":"5954","relation_version_is_last":true,"title":["Novel method for rapid in-situ hybridization of HER2 using non-contact alternating-current electric-field mixing"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-08-22T23:15:10.866410+00:00"}