@article{oai:air.repo.nii.ac.jp:00005844,
 author = {Umakoshi, Michinobu and Nakamura, Akiko and Tsuchie, Hiroyuki and Li, Zhuo and Kudo‑Asabe, Yukitsugu and Miyabe, Ken and Ito, Yukinobu and Yoshida, Makoto and Nagasawa, Hiroyuki and Okada, Kyoji and Nanjo, Hiroshi and Maeda, Daichi and Miyakoshi, Naohisa and Tanaka, Masamitsu and Goto, Akiteru},
 issue = {1},
 journal = {Scientific Reports},
 month = {Jan},
 note = {Even when treated comprehensively by surgery, chemotherapy, and radiotherapy, soft-tissue sarcoma has an unfavorable outcome. Because soft-tissue sarcoma is rare, it is the subject of fewer clinicopathological studies, which are important for clarifying pathophysiology. Here, we examined tumor-associated macrophages in the intratumoral and marginal areas of sarcomas to increase our knowledge about the pathophysiology. Seventy-five sarcoma specimens (not limited to a single histological type), resected at our institution, were collected, and the number of CD68-, CD163-, and CD204-positive macrophages in the intratumoral and marginal areas was counted. We then performed statistical analysis to examine links between macrophage numbers, clinical factors, and outcomes. A high number of macrophages positive for all markers in both areas was associated with worse disease-free survival (DFS). Next, we divided cases according to the FNCLCC classification (Grade 1 and Grades 2/3). In the Grade 1 group, there was no significant association between macrophage number and DFS. However, in the Grade 2/3 group, high numbers of CD163- and CD204-positive macrophages in the marginal area were associated with poor DFS. By contrast, there was no significant difference between the groups with respect to high or low numbers of CD68-, CD163-, or CD204-positive macrophages in the intratumoral area. Multivariate analysis identified the number of CD163- and CD204-positive macrophages in the marginal area as an independent prognostic factor. Macrophage numbers in the marginal area of soft-tissue sarcoma may better reflect clinical behavior.},
 title = {Macrophage numbers in the marginal area of sarcomas predict clinical prognosis},
 volume = {13},
 year = {2023}
}