{"created":"2023-07-25T10:25:16.630422+00:00","id":5517,"links":{},"metadata":{"_buckets":{"deposit":"d9c1d05f-72d2-463e-abf1-ca391c3705a9"},"_deposit":{"created_by":15,"id":"5517","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"5517"},"status":"published"},"_oai":{"id":"oai:air.repo.nii.ac.jp:00005517","sets":["590:629:630"]},"author_link":["15536","15537","15538","15539","15540"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageStart":"e74580","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"PLoS ONE"}]}]},"item_10001_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"The endoplasmic reticulum (ER) plays essential roles in protein folding and assembly of secretory proteins. ER-resident molecular chaperones and related enzymes assist in protein maturation by co-operated interactions and modifications. However, the folding/assembly of multimeric proteins is not well understood. Here, we show that the maturation of fibrinogen, a hexameric secretory protein (two trimers from a, b and c subunits), occurs in a stepwise manner. The ac complex, a precursor for the trimer, is retained in the ER by lectin-like chaperones, and the b subunit is incorporated into the ac complex immediately after translation. ERp57, a protein disulfide isomerase homologue, is involved in the hexamer formation from two trimers. Our results indicate that the fibrinogen hexamer is formed sequentially, rather than simultaneously, using kinetic pause by lectin chaperones. This study provides a novel insight into the assembly of most abundant multi-subunit secretory proteins.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"PLoS"}]},"item_10001_relation_25":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1371/journal.pone.0074580","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":" ©2013 Tamura et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1724099666130":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"1932-6203","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tamura, Taku","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"15536","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Arai, Seisuke","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"15537","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nagaya, Hisao","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"15538","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mizuguchi, Jun","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"15539","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wada, Ikuo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"15540","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-11-25"}],"displaytype":"detail","filename":"riA_2021_10.pdf","filesize":[{"value":"5.3 MB"}],"format":"application/pdf","licensefree":"CC BY","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"riA_2021_10","url":"https://air.repo.nii.ac.jp/record/5517/files/riA_2021_10.pdf"},"version_id":"e42f2a2c-c939-4a9a-93f8-3289333b5d8b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Stepwise Assembly of Fibrinogen Is Assisted by the Endoplasmic Reticulum Lectin-Chaperone System in HepG2 Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Stepwise Assembly of Fibrinogen Is Assisted by the Endoplasmic Reticulum Lectin-Chaperone System in HepG2 Cells","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"15","path":["630"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-11-25"},"publish_date":"2021-11-25","publish_status":"0","recid":"5517","relation_version_is_last":true,"title":["Stepwise Assembly of Fibrinogen Is Assisted by the Endoplasmic Reticulum Lectin-Chaperone System in HepG2 Cells"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-08-24T22:36:05.585621+00:00"}