@article{oai:air.repo.nii.ac.jp:00005424,
 author = {Fujishima, Satoshi and Imai, Kazuhiro and Nakamura, Ryuta and Nanjo, Hiroshi and Saito, Yoshitaro and Saito, Hajime and Terata, Kaori and Sato, Yusuke and Motoyama, Satoru and Akagami, Yoichi and Minamiya, Yoshihiro},
 journal = {Scientific Reports},
 month = {},
 note = {Echinoderm microtubule-associated protein-like 4 gene and anaplastic lymphoma kinase gene (EML4-ALK) rearrangement is a key driver mutation in non-small cell lung cancer (NSCLC). Although Break-Apart ALK fluorescence in situ hybridization (FISH) is a reliable diagnostic method for detecting ALK gene rearrangement, it is too costly and time-consuming for use as a routine screening test. Our aim was to evaluate the clinical utility of a novel rapid FISH (RaFISH) method developed to facilitate hybridization. RaFISH takes advantage of the non-contact mixing effect of an alternating current (AC) electric field. Eighty-five specimens were used from patients diagnosed with NSCLC identified immunohistochemically as ALK 0, (1/2+) or (3+). With RaFISH, the ALK test was completed within 4.5 h, as compared to 20 h needed for the standard FISH. Although RaFISH produced results more promptly, the staining and accuracy of the ALK evaluation with RaFISH was equal to the standard. We found 97.6% agreement between FISH and RaFISH based on the status of the ALK signals. These results suggest RaFISH could be used as a clinical tool to promptly determine ALK status.},
 title = {Novel method for rapid fluorescence in-situ hybridization of ALK rearrangement using non-contact alternating current electric field mixing},
 volume = {7:15116},
 year = {2017}
}