{"created":"2023-07-25T10:23:42.118369+00:00","id":3460,"links":{},"metadata":{"_buckets":{"deposit":"c20424cd-eb86-4973-ac9c-a08321157356"},"_deposit":{"created_by":15,"id":"3460","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"3460"},"status":"published"},"_oai":{"id":"oai:air.repo.nii.ac.jp:00003460","sets":["611:862:863:1198"]},"author_link":["11612","11613","11614","11615","11616","11617","11618","11619","11620"],"item_10001_biblio_info_7":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3/4","bibliographicPageEnd":"145","bibliographicPageStart":"139","bibliographicVolumeNumber":"45","bibliographic_titles":[{"bibliographic_title":"秋田医学","bibliographic_titleLang":"ja"},{"bibliographic_title":"Akita journal of medicine","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Human epidermal growth factor receptor 2 (HER2)-targeted agents are effective treatment for patients with HER2-positive breast cancer. However, the lack of survival benefit in HER2-negative patients as well as the toxic effects and high cost of these drugs highlight the need for accurate and prompt assessment of HER2 status. Our aim was to evaluate the clinical utility of a novel one-step, fully automated in-situ hybridization (Auto-RISH) method developed to facilitate hybridization. This method takes advantage of the non-contact mixing effect of an alternating current electric field. Eighteen specimens from patients diagnosed with primary breast cancer identified immunohistochemically as HER2 (2+) or (3+) were used. The specimens were all tested using DISH with an automated slide stainer (DISH-ASS), fluorescence in-situ hybridization, and Auto-RISH. With RISH the HER2 test was completed within 6 h, as compared to 20-22 h needed for the standard protocol. We found 88.8% agreement between standard DISH-ASS and Auto-RISH based on the status of the HER2 signals, and both methods stained nearly all samples from HER2-IHC 3+ patients equally well. These findings suggest Auto-RISH could potentially serve as an automated clinical tool for prompt determination of HER2 status in breast cancer samples.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_identifier_registration":{"attribute_name":"identifier_registration","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.20569/00003819","subitem_identifier_reg_type":"JaLC"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"秋田医学会","subitem_publisher_language":"ja"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1724099666130":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"0386-6106","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"AN00009294","subitem_source_identifier_type":"NCID"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mizusawa, Kaori","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"11612","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imai, Kazuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"11613","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nanjo, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"11614","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Akagami Yoichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"11615","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakamura, Ryuta","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"11616","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wakamatsu, Yuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"11617","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sato, Yusuke","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"11618","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Motoyama, Satoru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"11619","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Minamiya, Yoshihiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"11620","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-01"}],"displaytype":"detail","filename":"akitai45_3_4(139).pdf","filesize":[{"value":"754.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"akitai45_3_4(139).pdf","url":"https://air.repo.nii.ac.jp/record/3460/files/akitai45_3_4(139).pdf"},"version_id":"9723f6f8-7f25-4a13-abab-4550f73cc2d1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"breast cancer ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"HER2 ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"in-situ hybridization","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Development of novel one-step, fully automated in-situ hybridization of HER2 using non-contact alternating current electric field mixing","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of novel one-step, fully automated in-situ hybridization of HER2 using non-contact alternating current electric field mixing","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"15","path":["1198"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-07-01"},"publish_date":"2019-07-01","publish_status":"0","recid":"3460","relation_version_is_last":true,"title":["Development of novel one-step, fully automated in-situ hybridization of HER2 using non-contact alternating current electric field mixing"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-09-20T02:57:46.003968+00:00"}