@article{oai:air.repo.nii.ac.jp:00002981,
 author = {関, 信輔 and 場﨑, 恵太 and 小松, 幸恵 and 塚本, 智史 and 門脇, 歩 and 山口, 智和 and 幸丸, 純貴 and 福田, 康義 and 矢野, 愛美 and 小畑, 孝弘 and 小代田, 宗一 and 久場, 敬司 and 西島, 和俊 and Seki, Shinsuke and Basaki, Keita and Komatsu, Yukie and Tsukamoto, Satoshi and Kadowaki, Ayumi and Yamaguchi, Tomokazu and Kohmaru, Junki and Fukuda, Yasuyoshi and Yano, Manami and Obata, Takahiro and Koyota, Souichi and Kuba, Keiji and Nishijima, Kazutoshi},
 issue = {1},
 journal = {秋田医学, AKITA JOURNAL OF MADICINE},
 month = {Jun},
 note = {The CRISPR/Cas9 system is a powerful genome editing tool for generating knockout mice.　To generate mutant mice, single guide RNA （sgRNA） for tyrosinase was transcribed in vitro with template Oligo DNA and a mixture of either one or two sgRNAs and Cas9 mRNA were microinjected into the fertilized eggs of C57BL/6J（ black hairs and eyes）.　To determine whether tyrosinase gene is successfully knocked out, their phenotype（ black hairs/eyes or albino） was observed.　 In this study, to investigate whether knockout efficiency can be increased by microinjection of two sgRNAs, either one or two sgRNAs were microinjected.　Results indicated that when one sgRNA was microinjected, albino mice were obtained at relatively low efficiency （3/16, 19% or 13/23, 65%）; however, the efficiency was increased by microinjecting two sgRNAs （24/26 92.9%）.　In conclusion, we demonstrate here that knockout mice at F0 can be obtained by microinjection of two sgRNA and Cas9 mRNA at high efficiency.},
 pages = {37--43},
 title = {2種の guide RNA を同時に用いたCRISPR/Cas9 システムによる高率なノックアウトマウスの作出},
 volume = {44},
 year = {2017}
}