@article{oai:air.repo.nii.ac.jp:00002703,
 author = {SENOO, Haruki and WAKE, Kenjiro and MOMOI, Takashi and YOSHIKURA, Hiroshi},
 issue = {5},
 journal = {Biomedical Research},
 month = {},
 note = {We have previously demonstrated that HL-60 cells pretreated with 1α,25-dihydroxyvitamin
D3 at the stage of commitment were induced to differentiate into macrophage-
like cells by dimethyl sulfoxide (OMSO) at the stage of promotion (15). To
investigate the effect of OMSO on the differentiation of HL-60 cells induced by
1α,25-dihydroxyvitamin D3, three variant clones (ORHL-1, ORHL-2 and ORHL-3
cells) resistant to 1α,25-dihydroxyvitamin D3 were iso1α,ted from a OMSO-resistant
cell clone (ORHL) ofHL-60 cells. These variant clones were insusceptible to 1α,25-
dihydroxyvitamin D3 or OMSO, when based on the criteria of cell growth, expression
of antigens for human monocyte specific (OKMS) or human granulocyte specific
(80H5) monoclona1α,ntibodies, NBT-reducing activity, phagocytosis, nonspecific
esterase activity, and cathepsin Band 1α,ctivities. The variant clones, ORHL-1
and ORHL-3, which were insusceptible to 1α,25-dihydroxyvitamin D3 or OMSO
alone, were induced to differentiate into macrophage-like cells on simultaneous
treatment with 1α,25-dihydroxyvitamin D3 and OMSO. Furthermore, ORHL-1 but
not ORHL-3 differentiated into macrophage-like cells on I-day treatment with
1α,,25-dihydroxyvitamin D3 followed by 3-day treatment with OMSO. The other
variant, ORHL-2, did not differentiate with any combination of 1α,25-dihydroxyvitamin
D3 and OMSO. These results suggest that OMSO promotes the differentiation
of HL-60 cells initiated by 1α,,25-dihydroxyvitamin D3 into macrophages at
various stages of differentiation.},
 pages = {311--319},
 title = {ISOLATION AND CHARACTERIZATION OF CLONES OF A HUMAN LEUKEMIA CELL LINE (HL-60 CELLS) RESISTANT TO 1α,25-DIHYDROXYVITAMIN D3 AND DIMETHYL SULFOXIDE},
 volume = {7},
 year = {1986}
}