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G-protein-coupled estrogen recepter agonist suppresses airway inflammation in a mouse model of asthma through IL-10
http://hdl.handle.net/10295/2965
http://hdl.handle.net/10295/29659023512d-0fb2-4f1c-955d-664dd41a991b
| 名前 / ファイル | ライセンス | アクション |
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内容要旨及び審査結果要旨 (844.7 kB)
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本文 (241.1 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
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| 公開日 | 2015-12-04 | |||||||||
| タイトル | ||||||||||
| タイトル | G-protein-coupled estrogen recepter agonist suppresses airway inflammation in a mouse model of asthma through IL-10 | |||||||||
| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| 資源タイプ | doctoral thesis | |||||||||
| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 別タイトル | ||||||||||
| その他のタイトル | G 蛋白共役エストロゲン受容体アゴニストは喘息モデルマウスに対して IL-10 を介し気道炎症を抑制する | |||||||||
| 作成者 |
糸賀, 正道
× 糸賀, 正道
× ITOGA, Masamichi
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| 内容記述（抄録） | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | Estrogen influences the disease severity and sexual dimorphism in asthma, which is caused by complex mechanisms. Besides classical nuclear estrogen receptors (ERα/β), G-protein-coupled estrogen receptor (GPER) was recently established as an estrogen receptor on the cell membrane. Although GPER is associated with immunoregulatory functions of estrogen, the pathophysiological role of GPER in allergic inflammatory lung disease has not been examined. We investigated the effect of GPER-specific agonist G-1 in asthmatic mice. GPER expression in asthmatic lung was confirmed by immunofluorescent staining. OVA-sensitized BALB/c and C57BL/6 mice were treated with G-1 by daily subcutaneous injections during an airway challenge phase, followed by histological and biochemical examination. Strikingly, administration of G-1 attenuated airway hyperresponsiveness, accumulation of inflammatory cells, and levels of Th2 cytokines (IL-5 and IL-13) in BAL fluid. G-1 treatment also decreased serum levels of anti-OVA IgE antibodies. The frequency of splenic Foxp3+CD4+ regulatory T cells and IL-10-producing GPER+CD4+ T cells was significantly increased in G-1-treated mice. Additionally, splenocytes isolated from G-1-treated mice showed greater IL-10 production. G-1-induced amelioration of airway inflammation and IgE production were abolished in IL-10-deficient mice. Taken together, these results indicate that extended GPER activation negatively regulates the acute asthmatic condition by altering the IL-10-producing lymphocyte population. The current results have potential importance for understanding the mechanistic aspects of function of estrogen in allergic inflammatory response. |
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| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 書誌情報 | 発行日 2015-11-01 | |||||||||
| 出版者 | ||||||||||
| 出版者 | 秋田大学 | |||||||||
| 備考 | ||||||||||
| 秋田大学審査学位論文（PLoS ONE,10(8)掲載論文） | ||||||||||
| 学位名 | ||||||||||
| 学位名 | 博士（医学） | |||||||||
| 学位授与機関 | ||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||
| 学位授与機関識別子 | 11401 | |||||||||
| 学位授与機関名 | 秋田大学 | |||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2015-09-24 | |||||||||
| 学位授与番号 | ||||||||||
| 学位授与番号 | 甲第1139号 | |||||||||
