{"created":"2023-07-25T10:22:36.281587+00:00","id":2015,"links":{},"metadata":{"_buckets":{"deposit":"0132426e-f26d-49b7-b9c1-b0b3162c79cb"},"_deposit":{"created_by":3,"id":"2015","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2015"},"status":"published"},"_oai":{"id":"oai:air.repo.nii.ac.jp:00002015","sets":["611:862:863:937"]},"author_link":["7557","7550","7552","7554","7551","7555","7553","7556","7549"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-02-28","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"79","bibliographicPageStart":"73","bibliographicVolumeNumber":"39","bibliographic_titles":[{"bibliographic_title":"秋田医学"}]}]},"item_10001_description_5":{"attribute_name":"内容記述（抄録）","attribute_value_mlt":[{"subitem_description":"Nodal micrometastasis in non-small cell lung cancer (NSCLC) is associated with a poorer survival rate than node-negative disease. Furthermore, lymph node micrometastasis often cannot be detected using conventional hematoxylin and eosin staining of frozen sections ; detection requires additional time-consuming immunohistochemical (IHC) analysis of paraffin-embedded tissue. We previously developed and reported a device that enables us to complete IHC analyses within 11 minutes. In the present study, we used this rapid-IHC protocol with an anti-cytokeratin antibody and analyzed 205 mediastinal lymph nodes dissected during surgery for NSCLC. Although we modified the original rapid-IHC procedure to block endogenous peroxidase activity, which could potentially cause misdiagnosis, the staining was still completed within 19 min. On the basis of conventional histological examination, 7 lymph nodes from 3 patients were deemed positive for micrometastasis. By contrast using hematoxylin and eosin staining, 13 lymph nodes from 7 patients were diagnosed positive on the basis of cytokeratin-detection using routine-IHC, and the same 13 nodes were diagnosed positive on the basis of rapid-IHC. That is, all nodes deemed positive with the routine-IHC procedure were also positive with the rapid-IHC procedure. Assuming the results of the routine-IHC are correct, the sensitivity, specificity and accuracy of rapid-IHC are 100%, 100% and 100%, respectively. These findings demonstrate the utility of our rapid-IHC analysis for intraoperative diagnosis of micrometastasis. However, our findings are limited by the fact that we tested the method using a single antibody at a single institute. Further investigation in multicenter studies will be needed to confirm the utility of this method.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"秋田医学会"}]},"item_10001_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AN00009294","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03866106","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kawaharada, Yasushi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Minamiya, Yoshihiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nanjo, Hirosi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saito, Hajime"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Konno, Hayato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kagaya, Masam"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Akagami, Yo-ichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Motoyama, Satoru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ogawa, Jun-ichi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-16"}],"displaytype":"detail","filename":"akitai39(73).pdf","filesize":[{"value":"936.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"akitai39(73).pdf","url":"https://air.repo.nii.ac.jp/record/2015/files/akitai39(73).pdf"},"version_id":"ad1bac64-562a-473d-8760-a78a28d360da"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"non-small cell lung cancer","subitem_subject_scheme":"Other"},{"subitem_subject":"lymph node metastasis","subitem_subject_scheme":"Other"},{"subitem_subject":"lymph node micrometastasis","subitem_subject_scheme":"Other"},{"subitem_subject":"immunohistochemical staining","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"INTRAOPERATIVE DETECTION OF LYMPH NODE MICROMETASTASIS USING A RAPID IMMUNOHISTOCHEMICAL STAINING METHOD IN NON-SMALL CELL LUNG CANCER","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"INTRAOPERATIVE DETECTION OF LYMPH NODE MICROMETASTASIS USING A RAPID IMMUNOHISTOCHEMICAL STAINING METHOD IN NON-SMALL CELL LUNG CANCER"}]},"item_type_id":"10001","owner":"3","path":["937"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-05-29"},"publish_date":"2013-05-29","publish_status":"0","recid":"2015","relation_version_is_last":true,"title":["INTRAOPERATIVE DETECTION OF LYMPH NODE MICROMETASTASIS USING A RAPID IMMUNOHISTOCHEMICAL STAINING METHOD IN NON-SMALL CELL LUNG CANCER"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-25T11:33:30.957484+00:00"}