| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2009-11-27 |
| タイトル |
|
|
タイトル |
SCANNING ELECTRONMICROSCOPIC OBSERVATION OF MATRIX VESICLE FORMATION USING CULTURED HUMAN OSTEOBLASTIC SARCOMA CELLS(SAOS-2 CELLS), AND EFFECTS OF DEXAMETHASONE AND HUMAN PARATHYROID HORMONE ON MATRIX VESICLE FORMATION |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Matrix vesicle |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Bone mineralization |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Parathyroid hormone |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Scanning electronmicroscopy |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Osteoporosis |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 作成者 |
TAZAWA, Hiroshi
YAMADA, Shin
SHIMADA, Yoichi
|
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
In osseous tissue, osteoblasts form the extracellular matrix using type I collagen and non-collagenous proteins. Mineralized bone matrix is believed to result from calcium phosphate accumulating in matrix vesicles to be released from osteoblasts, then forming hydroxyapatite crystals that are deposited into collagen. Bone formation is regulated by various hormones, but assessing the effects of hormones on matrix mineralization has been difficult. The present study is the first to successfully and quantitatively assess matrix mineralization using SaOS-2 human osteoblastic sarcoma cells in vitro. This study also proved that mineralization is significantly elevated by incubating SaOS-2 cells with an appropriate concentration of hPTH in the presence of dexamethasone by histologically analyzing cells under scanning electronmicroscopy and biochemically analyzing cells using the 45Ca2+ radioactive isotope. These results will be very useful in the clinical application of hPTH for the treatment of steroid-induced osteoporosis in the future. |
|
言語 |
en |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 書誌情報 |
ja : 秋田医学
巻 35,
号 3/4,
p. 185-199,
発行日 2009-03-01
|
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
03866106 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00009294 |
| 出版者 |
|
|
出版者 |
秋田医学会 |
|
言語 |
ja |
p185-199.pdf (1.3 MB)
