@article{oai:air.repo.nii.ac.jp:00001562,
 author = {HASEGAWA, Hitoshi and SAITO, Takashi and FUJIWARA, Yoshimasa and KUROKAWA, Fukiko and [ほか]},
 issue = {3-4},
 journal = {秋田医学},
 month = {Dec},
 note = {To evaluate the effect of low molecular weight heparin (LMWH) on
cardiovascular remodeling induced by long term inhibition of nitric oxide synthase with
N-nitro-L-arginine methyl ester (L-NAME), 40 male Sprague-Dawley rats were randomly
divided into four groups: the control group (n=10) that received 0.9% salt
solution; the L-NAME group (n=10) that received 30 mg/kg/day of L-NAME, the
LMWH3000 group (n=10) that received 30 mg/kg/day of L-NAME and 4 mg/kg/day
of LMWH (molecular weight (M.W.) of 3000) and the LMWH6000 group (n= 10)
that received 30 mg/kg/day of L-NAME and 4 mg/kg/day of LMWH (M.W. 6000).
All agents, including saline, were administered intraperitoneally by implantation of
osmotic mini pumps. Systolic blood pressure was measured by the tail-cuff method on
treatment days 1,7, 14, and 28, and histological examination of harvested cardiac tissue
and coronary arteries was performed on day 28. Systolic blood pressure was greater in
the L-NAME, LMWH3000 and LMWH6000 groups when compared with the control
group. There were no significant differences in activated coagulation time (ACT)
when comparing the four groups. Medial smooth muscle cell proliferation and hypertrophy
and increases in perivascular and myocardial fibrosis were observed in the L-NAME
group compared with the control group. Further, these changes were inhibited by the
coadministration of LMWH3000 or LWMH6000. This study demonstrates that LMWH
prevents cardiovascular remodeling induced by L-NAME in rat hearts independent of its
anticoagulant activity. Low molecular weight heparin prevents cardiovascular remodeling},
 pages = {221--230},
 title = {Low Molecular Weight Heparin Prevents Cardiovascular Remodeling Induced by the Long-Term Inhibition of Nitric Oxide Synthase with N-Nitro-L-Arginine Methyl Ester in Rat Hearts},
 volume = {31},
 year = {2004}
}