{"created":"2023-07-25T10:22:15.196316+00:00","id":1558,"links":{},"metadata":{"_buckets":{"deposit":"7c431363-f334-4be8-9cdb-3939482ea026"},"_deposit":{"created_by":15,"id":"1558","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"1558"},"status":"published"},"_oai":{"id":"oai:air.repo.nii.ac.jp:00001558","sets":["611:862:863:866"]},"author_link":["5651"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-12-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3-4","bibliographicPageEnd":"193","bibliographicPageStart":"185","bibliographicVolumeNumber":"31","bibliographic_titles":[{"bibliographic_title":"秋田医学"}]}]},"item_10001_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Objective: The aim of present study is to clarify HPV-16 integration in cervical cancer cells using a novel PCR-based strategy. Study Design: The physical states of HPV-16 in cervical cancer cell lines were ascertained by long and accurate polymerase chain reaction (LA-PCR). HPV-16 integration in these cells was examined with a PCR-based protocol. Results: Using LA-PCR to detect a series of viral and flanking cellular sequences, the sites of viral integration were precisely determined. All viral copies were integrated and disrupted one or more chromosomal region in CaSki cells. Although open reading frames E6/7 were preferentially conserved in the cells, there was no other obvious preference for the sites of integration. This LA- PCR-based protocol was useful for determining the sites of viral integration not only in cell lines but also in clinical cancer samples. Conclusion: The method described in this report can precisely detect HPV integration using a small amount of DNA. Its application to precancerous cervical lesions may lead to early identification of patients at high risk for malignant transformation of cervical lesions.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"秋田医学会"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1724099666130":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"03866106","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AN00009294","subitem_source_identifier_type":"NCID"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SUZUKI, Yoichiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5651","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-16"}],"displaytype":"detail","filename":"akigaku31-34c.pdf","filesize":[{"value":"471.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"akigaku31-34c.pdf","url":"https://air.repo.nii.ac.jp/record/1558/files/akigaku31-34c.pdf"},"version_id":"727ce4e5-e979-4d5e-b559-4923376ef53b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"HPV","subitem_subject_scheme":"Other"},{"subitem_subject":"Integration","subitem_subject_scheme":"Other"},{"subitem_subject":"SiHa cell","subitem_subject_scheme":"Other"},{"subitem_subject":"CaSki cell","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of HPV-16 Integration in Cervical Cancer Cells Using Long and Accurate PCR (LA-PCR)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of HPV-16 Integration in Cervical Cancer Cells Using Long and Accurate PCR (LA-PCR)","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"15","path":["866"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-01-19"},"publish_date":"2009-01-19","publish_status":"0","recid":"1558","relation_version_is_last":true,"title":["Characterization of HPV-16 Integration in Cervical Cancer Cells Using Long and Accurate PCR (LA-PCR)"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-08-22T06:31:55.456682+00:00"}