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Characterization of HPV-16 Integration in Cervical Cancer Cells Using Long and Accurate PCR (LA-PCR)
http://hdl.handle.net/10295/1555
http://hdl.handle.net/10295/155521652090-2e7d-4088-b13d-aebc77682390
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
akigaku31-34c.pdf (471.9 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2009-01-19 | |||||||
| タイトル | ||||||||
| タイトル | Characterization of HPV-16 Integration in Cervical Cancer Cells Using Long and Accurate PCR (LA-PCR) | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | HPV | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Integration | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | SiHa cell | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | CaSki cell | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 作成者 |
SUZUKI, Yoichiro
× SUZUKI, Yoichiro
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| 内容記述 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Objective: The aim of present study is to clarify HPV-16 integration in cervical cancer cells using a novel PCR-based strategy. Study Design: The physical states of HPV-16 in cervical cancer cell lines were ascertained by long and accurate polymerase chain reaction (LA-PCR). HPV-16 integration in these cells was examined with a PCR-based protocol. Results: Using LA-PCR to detect a series of viral and flanking cellular sequences, the sites of viral integration were precisely determined. All viral copies were integrated and disrupted one or more chromosomal region in CaSki cells. Although open reading frames E6/7 were preferentially conserved in the cells, there was no other obvious preference for the sites of integration. This LA- PCR-based protocol was useful for determining the sites of viral integration not only in cell lines but also in clinical cancer samples. Conclusion: The method described in this report can precisely detect HPV integration using a small amount of DNA. Its application to precancerous cervical lesions may lead to early identification of patients at high risk for malignant transformation of cervical lesions. | |||||||
| 言語 | en | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 書誌情報 |
秋田医学 巻 31, 号 3-4, p. 185-193, 発行日 2004-12-01 |
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| 収録物識別子 | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 03866106 | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AN00009294 | |||||||
| 出版者 | ||||||||
| 出版者 | 秋田医学会 | |||||||
