@article{oai:air.repo.nii.ac.jp:00001549,
 author = {UENO, Yasuharu and TERADA, Kunihiko and NAGAI, Hirokazu and WATANABE, Go and [ほか]},
 issue = {2},
 journal = {秋田医学},
 month = {Sep},
 note = {Tissue engineering using somatic stem cells is a new and exciting technique
which has the potential to facilitate tissue reconstruction. Recent studies on tissue
engineering have indicated advantages in using collagen matrices to restitute the structure
of an organ. Here we have demonstrated the efficacy of transplantation of hepatic
stem-like (HSL) cells, a previously established liver epithelial cell line with potential for
differentiation, using type I collagen sponge. To this end, HSL cells were cultured and
transplanted into Nagase's analbuminemic rat with spongy type I collagen matrices.
Consequently, HSL was able to express both albumin and TAT after the culture in
collagen sponge. In addition, the levels of serum albumin in recipient rats were found
to increase up to 1.5 fold relative to controls after transplantation. These findings
suggest that HSL cells are able to differentiate into functional hepatocytes, and that
biodegradable collagen sponge may enhance hepatic differentiation of HSL cells by
providing an appropriate microenvironment.},
 pages = {113--119},
 title = {Application of Collagen Scaffolds for Hepatic Stem-Like Cell Transplantation},
 volume = {31},
 year = {2004}
}