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Modulation of Endothelial Cell Migration by Heparanase
http://hdl.handle.net/10295/1540
http://hdl.handle.net/10295/1540d36090e3-614d-4c2d-ab3a-33a35bc7f8ff
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
akigaku31-1h.pdf (576.6 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2009-01-18 | |||||||
| タイトル | ||||||||
| タイトル | Modulation of Endothelial Cell Migration by Heparanase | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Heparanase | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | HUVEC | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | FGF | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | VEGF | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | heparan sulfate proteoglycan | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 作成者 |
HORIKAWA, Naoki
× HORIKAWA, Naoki
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| 内容記述 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Cancer cells need new angiogenesis to grow and invade into new areas. A currently accepted model of angiogenesis associated with cancers involves growth factors, such as fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF), produced or induced by cancers stimulating endothelial cells in the surrounding tissue to from new blood vessels. Other molecules could also participate in the mechanism of angiogenesis by modulating growth factor activities. Heparan sulfate proteoglycan (HSPG) functions as one of such molecule by specifically binding to FGF and VEGF. Heparanase, an endo-fi-glucuronidase specifically degrading HSPG, has been reported in some cancers and implicated in the mechanism of metastasis. Since heparanase degrades HSPG and thus could modulate FGF and VEGF activities, the potential role of this enzyme in angiogenic mechanism was investigated. The migration of human umbilical vein endothelial cells (HUVECs) toward FGF and VEGF in the Boyden chamber was employed as a model system of angiogenesis. Addition of purified heparanase protein to the HUVEC culture significantly abrogated the FGF- and VEGF-induced cell migration. Reversal of this inhibitory activity of heparanase by heparin suggested that the effect involved degradation of HSPG. HUVEC transiently transfected with heparanase gene also exhibited suppressed migration toward FGF and VEGF. These results clearly demonstrated that heparanase in amounts sufficient to degrade cell surface HSPG abrogated the migration of HUVEC induced by FGF or VEGF_ The modulation of growth factor activities using heparanase may provide useful tools devising new cancer therapies. | |||||||
| 言語 | en | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 書誌情報 |
秋田医学 巻 31, 号 1, p. 83-93, 発行日 2004-05-01 |
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| 収録物識別子 | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 03866106 | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AN00009294 | |||||||
| 出版者 | ||||||||
| 出版者 | 秋田医学会 | |||||||
