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The Staining of Cortical Cytoplasmic Layer and the Behavior of PAS-Stained Granules in Sea Urchin Egg
http://hdl.handle.net/10295/992
http://hdl.handle.net/10295/9920ab3e8f7-284b-4802-aec2-5f36272ed1c4
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
yamamoto9.pdf (645.5 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||
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| 公開日 | 2008-07-24 | |||||||||
| タイトル | ||||||||||
| タイトル | The Staining of Cortical Cytoplasmic Layer and the Behavior of PAS-Stained Granules in Sea Urchin Egg | |||||||||
| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| 主題 | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | sea urchin | |||||||||
| 主題 | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Hemicentrotus pulcherrimus | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 作成者 |
YAMAMOTO, Masahiko
× YAMAMOTO, Masahiko
× 山本, 穆彦
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| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | Unfertilized and fertilized eggs at every developmental stages of the sea urchin, Hemicentrotus pulcherrimus, were first immersed in hypotonic sea water; then centrifugalized in that condition with a force of about 1300 x G for ten minutes. These eggs were fixed with Bouin's solution at each stage of development and sectioned with ordinary paraffin method. The sectioned preparations were stained with the Lillie's PAS method. By the centrifugation, the cortical cytoplasm was clearly observed "both in living and sectioned prepartions. The thickness calculated is about less than 4 tim. In this experiment, the behavior of the cytoplasmic granules stained with PAS method were discussed. |
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| 出版タイプ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 書誌情報 |
秋田大学教育学部研究紀要 自然科学 号 40, p. 9-14, 発行日 1989-02-01 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 03651649 | |||||||||
| NCID | ||||||||||
| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | AN00010282 | |||||||||
| 出版者 | ||||||||||
| 出版者 | 秋田大学教育学部 | |||||||||
